Desire Otsaghe Ekore, Larson Boundenga, Richard Onanga, Rolande Mabika Mabika, Serge Kumulungui Brice
Centre International de Recherche Médicales de Franceville, BP: 769, Franceville, Gabon.
Ecole Doctorale Régional d'Afrique Central, BP: 876, Franceville, Gabon.
Vet World. 2022 Mar;15(3):714-721. doi: 10.14202/vetworld.2022.714-721. Epub 2022 Mar 25.
The emergence of antibiotic resistance is a major problem worldwide. Antibiotics are often used to prevent or treat infections in livestock. This study aimed to investigate antibiotic resistance in enterococci in Gabonese livestock.
We collected 174 animal samples (46 laying hens, 24 swine, 62 cattle, and 42 sheep) from farms in four provinces of Gabon. Bacterial strains belonging to the genus were obtained using selective media and polymerase chain reaction targeting the gene. Antibiotic susceptibility was determined by the disk diffusion method on Mueller-Hinton agar.
Enterococci were present in 160 of the samples (97%), distributed as follows: laying hens (100%, 41/41), swine (100%, 22/22), small ruminants (88%, 37/42), and cattle (100%, 60/60). Resistance to cephalothin/cephalexin, streptomycin, and rifampicin (RIF) was high, and resistance to vancomycin (VAN), erythromycin, and tetracycline was moderate. A high diversity of resistance was found in Haut-Ogooué and Estuaire provinces. Laying hens and swine showed moderate levels of resistance to ciprofloxacin and penicillin, while sheep and cattle had high levels of resistance to RIF. All species showed a high level of resistance to VAN. We found various patterns of multiple resistances in the isolates, and the multiple resistance indexes ranged from 0.2 to 0.8.
This study shows that livestock in Gabon can be considered potential reservoirs of resistance.
抗生素耐药性的出现是全球范围内的一个主要问题。抗生素常用于预防或治疗家畜感染。本研究旨在调查加蓬家畜中肠球菌的抗生素耐药性。
我们从加蓬四个省份的农场收集了174份动物样本（46只蛋鸡、24头猪、62头牛和42只羊）。使用选择性培养基和针对基因的聚合酶链反应获得属于该属的细菌菌株。通过在穆勒-欣顿琼脂上的纸片扩散法测定抗生素敏感性。
160份样本（97%）中存在肠球菌，分布如下：蛋鸡（100%，41/41）、猪（100%，22/22）、小反刍动物（88%，37/42）和牛（100%，60/60）。对头孢噻吩/头孢氨苄、链霉素和利福平（RIF）的耐药性较高，对万古霉素（VAN）、红霉素和四环素的耐药性中等。在上奥果韦省和河口省发现了高度多样的耐药性。蛋鸡和猪对环丙沙星和青霉素表现出中等水平的耐药性，而绵羊和牛对RIF具有高水平的耐药性。所有物种对VAN均表现出高水平的耐药性。我们在分离株中发现了多种多重耐药模式，多重耐药指数范围为0.2至0.8。
本研究表明加蓬的家畜可被视为耐药性的潜在储存库。
