Sarafi Eleana, Tsouvaltzis Pavlos, Chatzissavvidis Christos, Siomos Anastasios, Therios Ioannis
Department of Horticulture, Aristotle University, 54124 Thessaloniki, Greece.
Department of Horticulture, Aristotle University, 54124 Thessaloniki, Greece.
Plant Physiol Biochem. 2017 Mar;112:173-182. doi: 10.1016/j.plaphy.2016.12.018. Epub 2016 Dec 23.
The objectives of this research were to test a possible involvement of melatonin (MEL) and resveratrol (RES) in restoring growth and to control boron (B) toxicity in peppers. The plants were subjected to four different nutrient solution treatments as following: 1) half-strength Hoagland's nutrient solution (Control), 2) half-strength Hoagland's nutrient solution+100 μM B (100 μMB), 3) half-strength Hoagland's nutrient solution+100 μM boron+100 μMresveratrol (100 μMRES), and 4) half-strength Hoagland's nutrient solution+100 μM B+1 μMmelatonin (1 μM MEL). Pepper plants subjected to B excess (100 μM) for 68 days (d) exhibited visible B toxicity symptoms, reduced rate of photosynthesis (Pn) and reduced dry weight (DW), while their leaf and fruit had the greatest increase of B concentration. The reduction of photosynthesis was restored, the reduction of DW was prevented, while the B leaf and fruit accumulation was moderated with the application of both 100 μMresveratrol (RES) and 1 μMmelatonin (MEL). Moreover, plants exposed to MEL and/or RES displayed no visible B toxicity symptoms. The present study revealed a novel role of MEL and/or RES in the adaptation of pepper plants to B excess based on plant growth, physiological and biochemical criteria.
本研究的目的是测试褪黑素（MEL）和白藜芦醇（RES）在恢复辣椒生长以及控制硼（B）毒性方面的潜在作用。将植株进行如下四种不同的营养液处理：1）半强度霍格兰营养液（对照），2）半强度霍格兰营养液 + 100 μM硼（100 μMB），3）半强度霍格兰营养液 + 100 μM硼 + 100 μM白藜芦醇（100 μMRES），4）半强度霍格兰营养液 + 100 μM硼 + 1 μM褪黑素（1 μM MEL）。遭受过量硼（100 μM）处理68天的辣椒植株表现出明显的硼中毒症状，光合作用速率（Pn）降低，干重（DW）减少，而其叶片和果实中的硼浓度增加最多。通过施用100 μM白藜芦醇（RES）和1 μM褪黑素（MEL），光合作用的降低得到恢复，干重的减少得到预防，同时叶片和果实中硼的积累得到缓解。此外，暴露于MEL和/或RES的植株没有表现出明显的硼中毒症状。本研究基于植物生长、生理和生化标准揭示了MEL和/或RES在辣椒植株适应硼过量方面的新作用。
