利用脂质结合蛋白结构域对不同膜区室中的脂质变化进行定量分析。
Quantifying lipid changes in various membrane compartments using lipid binding protein domains.
作者信息
Várnai Péter, Gulyás Gergő, Tóth Dániel J, Sohn Mira, Sengupta Nivedita, Balla Tamas
机构信息
Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
Section on Molecular Signal Transduction, Program for Developmental Neuroscience, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, United States; Department of Physiology, Faculty of Medicine, Semmelweis University, Budapest, Hungary.
出版信息
Cell Calcium. 2017 Jun;64:72-82. doi: 10.1016/j.ceca.2016.12.008. Epub 2016 Dec 31.
One of the largest challenges in cell biology is to map the lipid composition of the membranes of various organelles and define the exact location of processes that control the synthesis and distribution of lipids between cellular compartments. The critical role of phosphoinositides, low-abundant lipids with rapid metabolism and exceptional regulatory importance in the control of almost all aspects of cellular functions created the need for tools to visualize their localizations and dynamics at the single cell level. However, there is also an increasing need for methods to determine the cellular distribution of other lipids regulatory or structural, such as diacylglycerol, phosphatidic acid, or other phospholipids and cholesterol. This review will summarize recent advances in this research field focusing on the means by which changes can be described in more quantitative terms.
细胞生物学中最大的挑战之一是绘制各种细胞器膜的脂质组成图谱，并确定控制脂质在细胞区室之间合成和分布过程的确切位置。磷酸肌醇是一类含量低、代谢快且在控制细胞功能几乎所有方面具有特殊调节重要性的脂质，这就需要有工具来在单细胞水平上可视化它们的定位和动态变化。然而，对于确定其他具有调节或结构功能的脂质（如二酰基甘油、磷脂酸或其他磷脂和胆固醇）的细胞分布的方法需求也在日益增加。本综述将总结该研究领域的最新进展，重点关注能够以更定量的方式描述变化的方法。
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