Basiricò L, Morera P, Dipasquale D, Tröscher A, Bernabucci U
Dipartimento di Scienze e Agrarie e Forestali (DAFNE), Università degli Studi della Tuscia, via S. Camillo De Lellis, s.n.c, 01100 Viterbo, Italy.
BASF-SE, 67117 Ludwigshafen, Germany.
J Dairy Sci. 2017 Mar;100(3):2299-2309. doi: 10.3168/jds.2016-11729. Epub 2017 Jan 11.
Some in vitro and in vivo studies have demonstrated protective effects of conjugated linoleic acid (CLA) isomers against oxidative stress and lipid peroxidation. However, only a few and conflicting studies have been conducted showing the antioxidant potential of essential fatty acids. The objectives of the study were to compare the effects of CLA to other essential fatty acids on the thiol redox status of bovine mammary epithelia cells (BME-UV1) and their protective role against oxidative damage on the mammary gland by an in vitro study. The BME-UV1 cells were treated with complete medium containing 50 μM of cis-9,trans-11 CLA, trans-10,cis-12 CLA, α-linolenic acid, γ-linolenic acid, and linoleic acid. To assess the cellular antioxidant response, glutathione, NADPH, and γ-glutamyl-cysteine ligase activity were measured 48 h after addition of fatty acids (FA). Intracellular reactive oxygen species and malondialdehyde production were also assessed in cells supplemented with FA. Reactive oxygen species production after 3 h of HO exposure was assessed to evaluate and to compare the potential protection of different FA against HO-induced oxidative stress. All FA treatments induced an intracellular GSH increase, matched by high concentrations of NADPH and an increase of γ-glutamyl-cysteine ligase activity. Cells supplemented with FA showed a reduction in intracellular malondialdehyde levels. In particular, CLA isomers and linoleic acid supplementation showed a better antioxidant cellular response against oxidative damage induced by HO compared with other FA.
一些体外和体内研究已经证明共轭亚油酸（CLA）异构体对氧化应激和脂质过氧化具有保护作用。然而，关于必需脂肪酸抗氧化潜力的研究较少且相互矛盾。本研究的目的是通过体外研究比较CLA与其他必需脂肪酸对牛乳腺上皮细胞（BME-UV1）硫醇氧化还原状态的影响及其对乳腺氧化损伤的保护作用。用含有50μM顺式-9，反式-11 CLA、反式-10，顺式-12 CLA、α-亚麻酸、γ-亚麻酸和亚油酸的完全培养基处理BME-UV1细胞。为了评估细胞的抗氧化反应，在添加脂肪酸（FA）48小时后测量谷胱甘肽、NADPH和γ-谷氨酰半胱氨酸连接酶活性。还评估了补充FA的细胞内活性氧和丙二醛的产生。评估HO暴露3小时后的活性氧产生，以评估和比较不同FA对HO诱导的氧化应激的潜在保护作用。所有FA处理均诱导细胞内GSH增加，同时伴有高浓度的NADPH和γ-谷氨酰半胱氨酸连接酶活性增加。补充FA的细胞内丙二醛水平降低。特别是，与其他FA相比，CLA异构体和亚油酸补充剂对HO诱导的氧化损伤表现出更好的抗氧化细胞反应。
