Qin Tao, Ren Zhe, Huang Yifan, Song Yulong, Lin Dandan, Li Jian, Ma Yufang, Wu Xiuqin, Qiu Fuan, Xiao Qi
Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health in Fujian Province, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Int J Biol Macromol. 2017 Apr;97:287-298. doi: 10.1016/j.ijbiomac.2017.01.039. Epub 2017 Jan 12.
In this study, polysaccharides extracted from Hericium erinaceus were modified to obtain its nine selenium derivatives, sHEP-sHEP. Their structures were identified, yields and selenium contents were determined, the phenotypic and functional maturation of murine bone marrow-derived dendritic cells (DCs) and relevant mechanisms were compared taking unmodified HEP as control. The results revealed that the selenylation were successful. sHEP, sHEP and sHEP treatment of DCs increased their surface expression of MHC-II and CD86 and indicated that sHEP, sHEP and sHEP induced DC maturation. Furthermore, sHEP and sHEP also significantly decreased DCs endocytosis and significantly enhanced cytokine (IL-12 and IFN-γ) production. In line with TLR4 activation, sHEP increased the phosphorylation of ERK, p38, and JNK, and the nuclear translocation of p-c-Jun, p-CREB, and c-Fos. sHEP also activated NF-κB signaling, as evidenced by degradation of IκBα/β and nuclear translocation of p65 and p50. Together, these results suggest that sHEP is a strong immunostimulant.
在本研究中，对从猴头菇中提取的多糖进行修饰，以获得其九种硒衍生物，即sHEP - sHEP。对其结构进行了鉴定，测定了产率和硒含量，并以未修饰的HEP作为对照，比较了小鼠骨髓来源的树突状细胞（DCs）的表型和功能成熟情况及相关机制。结果表明硒化反应成功。用sHEP、sHEP和sHEP处理DCs可增加其MHC-II和CD86的表面表达，表明sHEP、sHEP和sHEP可诱导DC成熟。此外，sHEP和sHEP还显著降低了DCs的内吞作用，并显著增强了细胞因子（IL-12和IFN-γ）的产生。与TLR4激活一致，sHEP增加了ERK、p38和JNK的磷酸化，以及p-c-Jun、p-CREB和c-Fos的核转位。sHEP还激活了NF-κB信号通路，IκBα/β的降解以及p65和p50的核转位证明了这一点。总之，这些结果表明sHEP是一种强效免疫刺激剂。
