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硒修饰后玉米须多糖抗氧化及抗炎活性的增强

Enhancement of Antioxidative and Anti-Inflammatory Activities of Corn Silk Polysaccharides After Selenium Modification.

作者信息

Zheng Yu-Yun, Tong Xin-Yi, Zhang Da-Ying, Ouyang Jian-Ming

机构信息

Institute of Biomineralization and Lithiasis Research, College of Chemistry and Materials Science; Jinan University, Guangzhou, 510632, People's Republic of China.

出版信息

J Inflamm Res. 2024 Nov 1;17:7965-7991. doi: 10.2147/JIR.S467665. eCollection 2024.

DOI:10.2147/JIR.S467665
PMID:39502937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11537195/
Abstract

OBJECTIVE

This study aimed to study the effect of selenium modification on the bioactivity of corn silk polysaccharides, particularly its antioxidant and anti-inflammatory functions.

METHODS

HNO-NaSeO was used to selenize degraded corn silk polysaccharides (DCSP). The structure and physicochemical properties of DCSP and selenized corn silk polysaccharides (Se-DCSP) were characterized by inductively coupled plasma emission spectroscopy, Fourier-transform infrared, ultraviolet-visible spectroscopy, nuclear magnetic resonance, nanometer, scanning electron microscopy, and thermogravimetric analysis. The protective effects of DCSP and Se-DCSP on HK-2 cells damaged by nano-calcium oxalate and the changes of inflammatory factors were detected by laser confocal microscopy, flow cytometry, and fluorescence microscopy.

RESULTS

The selenium content of DCSP and Se-DCSP were 19.5 and 1226.7 μg/g, respectively. Compared with DCSP, Se-DCSP showed significantly improved biological activity, including the scavenging ability of various free radicals (increased by about 2-3 times), the intracellular reactive oxygen content (decreased by about 1.5 times), and the mitochondrial membrane potential (decreased by about 2.5 times). Moreover, cell viability and morphological recovery ability were improved. Compared with DCSP, Se-DCSP significantly down-regulated HK-2 cell inflammatory factors MCP-1 (about 1.7 times), NLRP3, and NO (about 1.5 times).

CONCLUSION

The antioxidant activity and the ability to down-regulate the expression of inflammatory factors of Se-DCSP were significantly enhanced compared with DCSP, and Se-DCSP can better protect HK-2 cells from oxidative damage, indicating that Se-DCSP has a stronger potential ability to inhibit kidney stone formation.

摘要

目的

本研究旨在探讨硒修饰对玉米须多糖生物活性的影响，特别是其抗氧化和抗炎功能。

方法

采用HNO₃-Na₂SeO₃对降解玉米须多糖（DCSP）进行硒化。通过电感耦合等离子体发射光谱、傅里叶变换红外光谱、紫外可见光谱、核磁共振、纳米粒度分析、扫描电子显微镜和热重分析对DCSP和硒化玉米须多糖（Se-DCSP）的结构和理化性质进行表征。通过激光共聚焦显微镜、流式细胞术和荧光显微镜检测DCSP和Se-DCSP对纳米草酸钙损伤的HK-2细胞的保护作用以及炎症因子的变化。

结果

DCSP和Se-DCSP的硒含量分别为19.5和1226.7μg/g。与DCSP相比，Se-DCSP的生物活性显著提高，包括对各种自由基的清除能力（提高约2-3倍）、细胞内活性氧含量（降低约1.5倍）和线粒体膜电位（降低约2.5倍）。此外，细胞活力和形态恢复能力也得到改善。与DCSP相比，Se-DCSP显著下调HK-2细胞炎症因子MCP-1（约1.7倍）、NLRP3和NO（约1.5倍）。

结论

与DCSP相比，Se-DCSP的抗氧化活性和下调炎症因子表达的能力显著增强，且Se-DCSP能更好地保护HK-2细胞免受氧化损伤，表明Se-DCSP具有更强的抑制肾结石形成潜力。

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