二硫键连接水凝胶的机械化学功能化
Mechanochemical functionalization of disulfide linked hydrogels.
作者信息
Lee Junmin, Silberstein Meredith N, Abdeen Amr A, Kim Sang Yup, Kilian Kristopher A
机构信息
Department of Materials Science and Engineering and Micro and Nanotechnology Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Department of Mechanical and Aerospace Engineering, Cornell University, Ithaca, New York 14853, USA.
出版信息
Mater Horiz. 2016;3(5):447-451. doi: 10.1039/C6MH00091F. Epub 2016 Jun 2.
Abstract
Poly(ethylene glycol) hydrogels with disulfide linkages are functionalized through applied force. Compression or tension induces bond rupture at the relatively weak disulfide linkages, which will subsequently react through Michael-type addition with an acceptor molecule within the gel. We demonstrate the utility of this approach by patterning cell adhesion proteins through compression of a lithographically structured stamp, where cells predominately adhere to the compressed regions.
摘要
具有二硫键的聚乙二醇水凝胶通过外力作用实现功能化。压缩或拉伸会导致相对较弱的二硫键断裂，随后这些断裂的键会通过迈克尔型加成反应与凝胶中的受体分子发生反应。我们通过对光刻结构化印章进行压缩来对细胞粘附蛋白进行图案化，从而证明了这种方法的实用性，在这种情况下，细胞主要粘附在压缩区域。
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