Khazal Kamel F, Hill Donald L
Department of Biomedical Sciences, School of Veterinary Medicine, Tuskegee University, Tuskegee, Alabama 36088, USA.
Division of Preventive Medicine, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA.
J Cancer Metastasis Treat. 2015 Jul;1(2):94-100. doi: 10.4103/2394-4722.157601. Epub 2015 Jul 15.
The aim was to examine the anti-proliferative effect of a (WS) root extract in cell cultures and nude mouse xenografts of breast cancer cell line MDA-MB-231.
WS root extract was used to treat tumor cells at concentrations up to 100 µg and for nude mouse experiments, the mice received daily WS at 300 mg/kg by oral gavage for 8 weeks.
The WS extract reduced viability of MDA-MB-231 cells by 75% and 88% after exposure of the cells to 50 and 100 µg/mL, respectively, compared to vehicle-treated controls. WS extract caused a dose-dependent increase in the percentage of cells in the sub-G1 phase compared to untreated controls by 6% and 10% after exposure to 25 and 50 µg/mL WS extract, respectively. WS extract also inhibited proliferation of xenografted MDA-MB-231 cells. The WS extract caused reductions in xenograft size by 60% compared to the untreated control after 8 weeks of treatment. Six of ten mice in the control group showed tumor metastasis to the lung, whereas there was none in the mice treated with the WS extract. At the gene level, WS caused a 75% reduction in chemokine CCL2 expression ( < 0.05) in the xenografted tumors of the treated mice.
WS root extract inhibited proliferation of breast cancer cells and and significantly reduced expression of the cytokine, CCL2. These results warrant further studies to assess the underlying molecular mechanism of the anti-tumor activity of the WS extract in breast cancer.
本研究旨在检测某（WS）根提取物对乳腺癌细胞系MDA-MB-231细胞培养物及裸鼠异种移植瘤的抗增殖作用。
采用WS根提取物以高达100μg的浓度处理肿瘤细胞，在裸鼠实验中，小鼠每天经口灌胃给予300mg/kg的WS，持续8周。
与溶剂处理的对照组相比，将细胞分别暴露于50和100μg/mL的WS提取物后，WS提取物使MDA-MB-231细胞的活力分别降低了75%和88%。与未处理的对照组相比，分别暴露于25和50μg/mL的WS提取物后，WS提取物使处于亚G1期的细胞百分比呈剂量依赖性增加，分别增加了6%和10%。WS提取物还抑制了异种移植的MDA-MB-231细胞的增殖。治疗8周后，与未处理的对照组相比，WS提取物使异种移植瘤的大小减小了60%。对照组的10只小鼠中有6只出现肿瘤转移至肺部，而经WS提取物处理的小鼠中未出现肿瘤转移。在基因水平上，WS使治疗小鼠异种移植瘤中趋化因子CCL2的表达降低了75%（P<0.05）。
WS根提取物抑制了乳腺癌细胞的增殖，并显著降低了细胞因子CCL2的表达。这些结果值得进一步研究，以评估WS提取物在乳腺癌中抗肿瘤活性的潜在分子机制。
