Lin Na, Mo Xiaoli, Yang Yang, Zhang Hong
School of Food Science and Biological Engineering, Zhejiang Gongshang University, Hangzhou, Zhejiang Province, 310035, People's Republic of China.
Glycoconj J. 2017 Apr;34(2):241-253. doi: 10.1007/s10719-016-9759-y. Epub 2017 Jan 14.
Chondroitin sulfate (CS) and dermatan sulfate (DS) were extracted and purified from skins or bones of salmon (Salmo salar), snakehead (Channa argus), monkfish (Lophius litulon) and skipjack tuna (Katsuwonus pelamis). Size, structural sequences and sulfate groups of oligosaccharides in the purified CS and DS could be characterized and identified using high performance liquid chromatography (HPLC) combined with Orbitrap mass spectrometry. CS and DS chain structure varies depending on origin, but motif structure appears consistent. Structures of CS and DS oligosaccharides with different size and sulfate groups were compared between fishes and other animals, and results showed that some minor differences of special structures could be identified by hydrophilic interaction chromatography-liquid chromatography-fourier transform-mass/mass spectrometry (HILIC-LC-FT-MS/MS). For example, data showed that salmon and skipjack CS had a higher percentage content of high-level sulfated oligosaccharides than that porcine CS. In addition, structural information of different origins of CS and DS was analyzed by principal component analysis (PCA) and results showed that CS and DS samples could be differentiated according to their molecular conformation and oligosaccharide fragments information. Understanding CS and DS structure derived from different origins may lead to the production of CS or DS with unique disaccharides or oligosaccharides sequence composition and biological functions.
从鲑鱼（Salmo salar）、乌鳢（Channa argus）、安康鱼（Lophius litulon）和鲣鱼（Katsuwonus pelamis）的皮肤或骨骼中提取并纯化了硫酸软骨素（CS）和硫酸皮肤素（DS）。使用高效液相色谱（HPLC）结合Orbitrap质谱，可以对纯化后的CS和DS中寡糖的大小、结构序列和硫酸基团进行表征和鉴定。CS和DS的链结构因来源而异，但基序结构似乎一致。比较了鱼类与其他动物之间不同大小和硫酸基团的CS和DS寡糖结构，结果表明，通过亲水相互作用色谱-液相色谱-傅里叶变换-质谱/质谱（HILIC-LC-FT-MS/MS）可以识别一些特殊结构的微小差异。例如，数据显示鲑鱼和鲣鱼的CS中高硫酸化寡糖的含量百分比高于猪CS。此外，通过主成分分析（PCA）分析了不同来源的CS和DS的结构信息，结果表明CS和DS样品可以根据其分子构象和寡糖片段信息进行区分。了解源自不同来源的CS和DS结构可能会导致生产出具有独特二糖或寡糖序列组成及生物学功能的CS或DS。
