Wang Jiao, Wang Yu-Mei, Li Long-Yan, Chi Chang-Feng, Wang Bin
Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, China.
National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan, China.
Front Nutr. 2022 Jan 21;8:813780. doi: 10.3389/fnut.2021.813780. eCollection 2021.
For using aquatic by-products to manufacture high-value products, Skipjack tuna () roes were degreased, pretreated with microwave, and hydrolyzed using five proteases. The protein hydrolysate (TRPH) generated using Flavourzyme displayed the strongest 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Twelve antioxidative peptides were prepared from TRPH by ultrafiltration and chromatography methods and determined to be SGE, VDTR, AEM, QDHKA, TVM, QEAE, YEA, VEP, AEHNH, QEP, QAEP, and YVM with molecular weights of 291.24, 489.50, 349.41, 597.59, 349.44, 475.42, 381.36, 343.37, 606.58, 372.35, 443.42, and 411.49 Da, respectively. AEM, QDHKA, YEA, AEHNH, and YVM presented the strongest scavenging activity on DPPH radical (EC values of 0.250±0.035, 0.279±0.017, 0.233±0.012, 0.334±0.011, and 0.288±0.015 mg/ml, respectively), hydroxyl radical (EC values of 0.456±0.015, 0.536±0.021, 0.476 ± 0.051, 0.369 ± 0.052, and 0.413 ± 0.019 mg/ml, respectively), and superoxide anion free radical (EC values of 0.348 ± 0.018, 0.281 ± 0.013, 0.305 ± 0.022, 0.198 ± 0.011, and 0.425 ± 0.021 mg/ml, respectively). Moreover, AEM, QDHKA, YEA, AEHNH, and YVM presented high lipid peroxidation inhibition ability, Ferric-reducing power, and significant protective function on HO-induced Chang liver cells. Therefore, AEM, QDHKA, YEA, AEHNH, and YVM could be natural antioxidant ingredients used in pharmaceutical and functional products.
为了利用水产副产品制造高价值产品，对鲣鱼（）鱼籽进行脱脂、微波预处理，并使用五种蛋白酶进行水解。使用风味酶产生的蛋白质水解物（TRPH）表现出最强的2,2-二苯基-1-苦基肼基（DPPH）自由基清除活性。通过超滤和色谱方法从TRPH中制备了12种抗氧化肽，分别确定为SGE、VDTR、AEM、QDHKA、TVM、QEAE、YEA、VEP、AEHNH、QEP、QAEP和YVM，分子量分别为291.24、489.50、349.41、597.59、349.44、475.42、381.36、343.37、606.58、372.35、443.42和411.49 Da。AEM、QDHKA、YEA、AEHNH和YVM对DPPH自由基（EC值分别为0.250±0.035、0.279±0.017、0.233±0.012、0.334±0.011和0.288±0.015 mg/ml）、羟基自由基（EC值分别为0.456±0.015、0.536±0.021、0.476±0.051、0.369±0.052和0.413±0.019 mg/ml）和超氧阴离子自由基（EC值分别为0.348±0.018、0.281±0.013、0.305±0.022、0.198±0.011和0.425±0.021 mg/ml）表现出最强的清除活性。此外，AEM、QDHKA、YEA、AEHNH和YVM表现出高脂质过氧化抑制能力、铁还原能力以及对HO诱导的Chang肝细胞的显著保护作用。因此，AEM、QDHKA、YEA、AEHNH和YVM可作为天然抗氧化成分用于制药和功能性产品。
