Belyaev Anatoly A, Shternshis Margarita V, Chechenina Nina S, Shpatova Tatyana V, Lelyak Anastasya A
Department of Plant Protection, Novosibirsk State Agrarian University, Dobrolubov 160, Novosibirsk, Russia, 630039.
Environ Sci Pollut Res Int. 2017 Mar;24(8):7016-7022. doi: 10.1007/s11356-017-8427-5. Epub 2017 Jan 14.
In geographical locations with a short vegetative season and continental climate that include Western Siberia, growing primocane fruiting raspberry varieties becomes very important. However, it is necessary to help the plants to overcome the environmental stress factors. This study aimed to evaluate the impact of the pre-planting treatment of primocane fruiting raspberry root system with Bacillus strains on the following plant development under variable environmental conditions. In 2012, Bacillus subtilis RCAM В-10641, Bacillus amyloliquefaciens RCAM В-10642, and Bacillus licheniformis RCAM В-10562 were used for inoculating the root system of primocane fruiting raspberry cultivar Nedosyagaemaya before planting. The test suspensions were 10 CFU/ml for each bacterial strains. The effects of this treatment on plant growth and crop productivity were estimated in 2012-2015 growing seasons differed by environmental conditions. The pre-planting treatment by the bacterial strains increased the number of new raspberry canes and the number of plant generative organs as well as crop productivity compared to control. In addition, these bacilli acted as the standard humic fertilizer. Variable environmental factors such as air temperature, relative humidity, and winter and spring frosts seriously influenced the plant biological parameters and crop productivity of control plants. At the same time, the pre-planting primocane fruiting root treatment by Bacillus strains decreased the negative effects of abiotic stresses on plants in all years of the research. Of the three strains studied, B. subtilis was shown to reveal the best results in adaptation of primocane fruiting raspberry plants to environmental factors in Western Siberia. For the first time, the role of Bacillus strains in enhancing frost resistance in primocane fruiting raspberry plants was shown. These bacilli are capable of being the basis of multifunctional biological formulations for effective plant and environmental health management in growing primocane fruiting raspberry.
在包括西西伯利亚在内的生长季节较短且具有大陆性气候的地理位置，种植一年生结果型树莓品种变得非常重要。然而，有必要帮助植株克服环境胁迫因素。本研究旨在评估用芽孢杆菌菌株对一年生结果型树莓根系进行种植前处理，在可变环境条件下对植株后续生长发育的影响。2012年，使用枯草芽孢杆菌RCAM В - 10641、解淀粉芽孢杆菌RCAM В - 10642和地衣芽孢杆菌RCAM В - 10562在种植前对一年生结果型树莓品种Nedosyagaemaya的根系进行接种。每个细菌菌株的测试悬浮液浓度为10 CFU/ml。在2012 - 2015年生长季节，评估了这种处理对植株生长和作物生产力的影响，各年环境条件不同。与对照相比，细菌菌株的种植前处理增加了新树莓茎的数量、植株生殖器官的数量以及作物生产力。此外，这些芽孢杆菌起到了标准腐殖质肥料的作用。可变环境因素如气温、相对湿度以及冬季和春季霜冻严重影响了对照植株的生物学参数和作物生产力。与此同时，在研究的所有年份中，用芽孢杆菌菌株对一年生结果型树莓根系进行种植前处理都降低了非生物胁迫对植株的负面影响。在所研究的三种菌株中，枯草芽孢杆菌在一年生结果型树莓植株适应西西伯利亚环境因素方面表现出最佳效果。首次表明了芽孢杆菌菌株在增强一年生结果型树莓植株抗冻性方面的作用。这些芽孢杆菌能够成为用于一年生结果型树莓种植中有效管理植株和环境健康的多功能生物制剂的基础。
