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两个基因座,即 和 ,对 中的年度结果实性状有贡献。

Two Loci, and , Contribute to the Annual-Fruiting Trait in .

作者信息

Jibran Rubina, Spencer Jessica, Fernandez Gina, Monfort Amparo, Mnejja Mourad, Dzierzon Helge, Tahir Jibran, Davies Kevin, Chagné David, Foster Toshi M

机构信息

The New Zealand Institute for Plant & Food Research Limited, Palmerston North Research Centre, Palmerston North, New Zealand.

Department of Horticultural Science, North Carolina State University, Raleigh, NC, United States.

出版信息

Front Plant Sci. 2019 Oct 25;10:1341. doi: 10.3389/fpls.2019.01341. eCollection 2019.

DOI:10.3389/fpls.2019.01341
PMID:31708950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6824294/
Abstract

Most species have a biennial cycle of flowering and fruiting with an intervening period of winter dormancy, in common with many perennial fruit crops. Annual-fruiting (AF) varieties of raspberry ( and L.) and blackberry ( subgenus ) are able to flower and fruit in one growing season, without the intervening dormant period normally required in biennial-fruiting (BF) varieties. We used a red raspberry () population segregating for AF obtained from a cross between NC493 and 'Chilliwack' to identify genetic factors controlling AF. Genotyping by sequencing (GBS) was used to generate saturated linkage maps in both parents. Trait mapping in this population indicated that AF is controlled by two newly identified loci ( and ) located on linkage groups (LGs) 3 and 4. The location of these loci was analyzed using single-nucleotide polymorphism (SNP) markers on independent red raspberry and blackberry populations segregating for the AF trait. This confirmed that AF in is regulated by loci on LG 3 and 4, in addition to a previously reported locus on LG 7. Comparative RNAseq analysis at the time of floral bud differentiation in an AF and a BF variety revealed candidate genes potentially regulating the trait.

摘要

与许多多年生果树作物一样,大多数物种具有两年一次的开花结果周期,并伴有冬季休眠期。树莓(悬钩子属)和黑莓(黑莓亚属)的一年生结果(AF)品种能够在一个生长季节内开花结果,无需两年生结果(BF)品种通常所需的中间休眠期。我们利用从NC493和“奇利瓦克”杂交后代中分离得到的一个AF红树莓群体来鉴定控制AF的遗传因素。通过测序进行基因分型(GBS)用于构建两个亲本的饱和连锁图谱。该群体的性状定位表明,AF受位于连锁群(LG)3和4上的两个新鉴定的基因座(AF1和AF2)控制。利用独立的红树莓和黑莓群体中分离的AF性状的单核苷酸多态性(SNP)标记对这些基因座的位置进行了分析。这证实了树莓中的AF除了受LG 7上先前报道的一个基因座调控外,还受LG 3和4上的基因座调控。对一个AF品种和一个BF品种花芽分化时的比较RNA测序分析揭示了可能调控该性状的候选基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/a30050069d60/fpls-10-01341-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/ffdee153e424/fpls-10-01341-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/5b433b49ba22/fpls-10-01341-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/e99be0c3e917/fpls-10-01341-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/74c113258ead/fpls-10-01341-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/230a83ac2bec/fpls-10-01341-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/adf18c095c15/fpls-10-01341-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/a30050069d60/fpls-10-01341-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/ffdee153e424/fpls-10-01341-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/5b433b49ba22/fpls-10-01341-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/e99be0c3e917/fpls-10-01341-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/74c113258ead/fpls-10-01341-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/230a83ac2bec/fpls-10-01341-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/adf18c095c15/fpls-10-01341-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a486/6824294/a30050069d60/fpls-10-01341-g007.jpg

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