Babu Anish, Muralidharan Ranganayaki, Amreddy Narsireddy, Mehta Meghna, Munshi Anupama, Ramesh Rajagopal
IEEE Trans Nanobioscience. 2016 Dec;15(8):849-863. doi: 10.1109/TNB.2016.2621730.
Gene silencing through RNA interference (RNAi) has emerged as a potential strategy in manipulating cancer causing genes by complementary base-pairing mechanism. Small interfering RNA (siRNA) is an important RNAi tool that has found significant application in cancer therapy. However due to lack of stability, poor cellular uptake and high probability of loss-of-function due to degradation, siRNA therapeutic strategies seek safe and efficient delivery vehicles for in vivo applications. The current review discusses various nanoparticle systems currently used for siRNA delivery for cancer therapy, with emphasis on liposome based gene delivery systems. The discussion also includes various methods availed to improve nanoparticle based-siRNA delivery with target specificity and superior efficiency. Further this review describes challenges and perspectives on the development of safe and efficient nanoparticle based-siRNA-delivery systems for cancer therapy.
通过RNA干扰（RNAi）实现的基因沉默已成为一种通过互补碱基配对机制操纵致癌基因的潜在策略。小干扰RNA（siRNA）是一种重要的RNAi工具，在癌症治疗中已得到广泛应用。然而，由于缺乏稳定性、细胞摄取能力差以及降解导致功能丧失的可能性高，siRNA治疗策略需要寻找安全有效的递送载体用于体内应用。本综述讨论了目前用于癌症治疗的siRNA递送的各种纳米颗粒系统，重点是基于脂质体的基因递送系统。讨论还包括为提高基于纳米颗粒的siRNA递送的靶向特异性和效率而采用的各种方法。此外，本综述描述了开发用于癌症治疗的安全有效的基于纳米颗粒的siRNA递送系统的挑战和前景。
