Song Yan, Peng Changliang, Lv Shasha, Cheng Jing, Liu Shanshan, Wen Qing, Guan Guangju, Liu Gang
Department of Nephrology, The Second Hospital of Shandong University, Shandong University, Jinan, China.
Department of Spinal Surgery, The Second Hospital of Shandong University, Shandong University, Jinan, China.
Int Immunopharmacol. 2017 Mar;44:115-122. doi: 10.1016/j.intimp.2017.01.008. Epub 2017 Jan 13.
Adipose-derived stem cells (ADSCs) have been successfully used to treat acute kidney injury or acute renal failure. However, the effect of ADSCs on treating renal interstitial fibrosis remains unknown. Here, we assessed the therapeutic efficacy of ADSCs on renal interstitial fibrosis induced by unilateral ureter obstruction (UUO) and explored the potential mechanisms. After 7days of UUO, rats were injected with ADSCs (5×10) or vehicle via tail vein. We found that ADSCs administration significantly ameliorated renal interstitial fibrosis, the occurrence of epithelial-mesenchymal transition (EMT) and inflammatory response. Furthermore, ADSCs administration could inhibit the activation of transforming growth factor-β1 (TGF-β1) signaling pathway, which might play a crucial role in renal interstitial fibrosis of the UUO model rats. These results suggested that ADSCs treatment attenuates renal interstitial fibrosis possibly through inhibition of EMT and inflammatory response via TGF-β1 signaling pathway. Therefore, ADSCs may be an effective therapeutic strategy for the treatment of renal interstitial fibrosis.
脂肪来源干细胞（ADSCs）已成功用于治疗急性肾损伤或急性肾衰竭。然而，ADSCs对治疗肾间质纤维化的效果仍不清楚。在此，我们评估了ADSCs对单侧输尿管梗阻（UUO）诱导的肾间质纤维化的治疗效果，并探讨了潜在机制。UUO 7天后，通过尾静脉给大鼠注射ADSCs（5×10）或赋形剂。我们发现，给予ADSCs可显著改善肾间质纤维化、上皮-间质转化（EMT）的发生及炎症反应。此外，给予ADSCs可抑制转化生长因子-β1（TGF-β1）信号通路的激活，这可能在UUO模型大鼠的肾间质纤维化中起关键作用。这些结果表明，ADSCs治疗可能通过TGF-β1信号通路抑制EMT和炎症反应来减轻肾间质纤维化。因此，ADSCs可能是治疗肾间质纤维化的一种有效治疗策略。
