Amirkalali Bahareh, Sohrabi Masoud Reza, Esrafily Ali, Jalali Mahmoud, Gholami Ali, Hosseinzadeh Payam, Keyvani Hossein, Shidfar Farzad, Zamani Farhad
Gastrointestinal and Liver Disease Research Center (GILDRC), Iran University of Medical Sciences, Tehran, Iran.
Med Princ Pract. 2017;26(3):251-257. doi: 10.1159/000455862. Epub 2017 Jan 9.
This study explored the association between serum nicotinamide phosphoribosyltransferase (NAMPT) and hepatic de novo lipogenesis (DNL) in nonalcoholic fatty liver disease (NAFLD) and determined whether or not this association is sex dependent.
In this cross-sectional study, 62 consecutive patients (32 males, 30 females) with NAFLD were recruited. Serum NAMPT (by ELISA), palmitic acid, and the DNL index of erythrocyte membranes as markers of hepatic DNL (by gas chromatography) were analyzed. The controlled attenuation parameter (CAP) and body impedance analyzer were used to assess hepatic and body fat, respectively. Univariate and multiple linear regressions (to adjust for confounders) were used to analyze the association of serum NAMPT with palmitic acid, DNL index, CAP, and body fat.
The respective values of serum NAMPT (2.44 ± 1.03 vs. 2.45 ± 1.13 ng/mL, p = 0.98), DNL index (3.11 [2.60-3.71] vs. 3.05 [2.40-3.59], p = 0.90), and palmitic acid (20.55% [15.34-24.04] vs. 22.64% [21.15-25.95], p = 0.07) were not significantly different between men and women, but those of CAP (326 [300-340] vs. 300 [261.25-329], p = 0.002) and body fat (37.71 ± 3.80 vs. 26.60 ± 5.70, p < 0.001) were significantly higher in women. In women, serum NAMPT had a significant negative association with the DNL index (β = -0.56, p = 0.01). The DNL index also had a significant negative association with body fat (β = -0.46, p = 0.02). In men, the only significant association was the positive association between serum NAMPT and CAP (β = 0.35, p = 0.035).
Higher serum NAMPT in women was associated with a lower hepatic DNL index, while in men it was associated with higher hepatic fat and had no association with the DNL index. Therefore, the serum NAMPT level interpretation for NAFLD prognosis is probably sex dependent.
本研究探讨非酒精性脂肪性肝病（NAFLD）患者血清烟酰胺磷酸核糖转移酶（NAMPT）与肝脏从头脂肪生成（DNL）之间的关联，并确定这种关联是否存在性别差异。
在这项横断面研究中，连续招募了62例NAFLD患者（男性32例，女性30例）。分析血清NAMPT（采用酶联免疫吸附测定法）、棕榈酸以及作为肝脏DNL标志物的红细胞膜DNL指数（采用气相色谱法）。分别使用受控衰减参数（CAP）和人体阻抗分析仪评估肝脏脂肪和身体脂肪。采用单因素和多因素线性回归（以调整混杂因素）分析血清NAMPT与棕榈酸、DNL指数、CAP和身体脂肪之间的关联。
血清NAMPT（2.44±1.03对2.45±1.13 ng/mL，p = 0.98）、DNL指数（3.11 [2.60 - 3.71]对3.05 [2.40 - 3.59]，p = 0.90）和棕榈酸（20.55% [15.34 - 24.04]对22.64% [21.15 - 25.95]，p = 0.07）在男性和女性之间无显著差异，但CAP（326 [300 - 340]对300 [261.25 - 329]，p = 0.002）和身体脂肪（37.71±3.80对26.60±5.70，p < 0.001）在女性中显著更高。在女性中，血清NAMPT与DNL指数呈显著负相关（β = -0.56，p = 0.01）。DNL指数也与身体脂肪呈显著负相关（β = -0.46，p = 0.02）。在男性中，唯一显著的关联是血清NAMPT与CAP之间的正相关（β = 0.35，p = 0.035）。
女性血清NAMPT水平较高与较低的肝脏DNL指数相关，而男性血清NAMPT水平较高与较高的肝脏脂肪相关，且与DNL指数无关。因此，NAFLD预后的血清NAMPT水平解读可能存在性别差异。
