Toraskar Jimita, Magnussen Synnøve N, Chawla Konika, Svineng Gunbjørg, Steigedal Tonje S
Department of Clinical and Molecular Medicine Faculty of Medicine and Health Sciences Norwegian University of Science and Technology (NTNU) Trondheim Norway.
Central Norway Regional Health Authority Stjørdal Norway.
FEBS Open Bio. 2018 Nov 15;8(12):1992-2001. doi: 10.1002/2211-5463.12544. eCollection 2018 Dec.
Nephronectin (NPNT) is an extracellular matrix (ECM) protein involved in kidney development. We recently reported intracellular NPNT as a potential prognostic marker in breast cancer and that NPNT promotes metastasis in an integrin-dependent manner. Here, we used reverse-phase protein array (RPPA) to analyze NPNT-triggered intracellular signaling in the 66cl4 mouse breast cancer cell line. The results showed that the integrin-binding enhancer motif is important for the cellular effects upon NPNT interaction with its receptors, including phosphorylation of p38 mitogen-activated protein kinase (MAPK). Furthermore, analysis using prediction tools suggests involvement of NPNT in promoting cell viability. In conclusion, our results indicate that NPNT, via its integrin-binding motifs, promotes cell viability through phosphorylation of p38 MAPK.
肾连蛋白（NPNT）是一种参与肾脏发育的细胞外基质（ECM）蛋白。我们最近报道，细胞内NPNT作为乳腺癌的一种潜在预后标志物，且NPNT以整合素依赖的方式促进转移。在此，我们使用反相蛋白质阵列（RPPA）分析66cl4小鼠乳腺癌细胞系中NPNT触发的细胞内信号传导。结果表明，整合素结合增强基序对于NPNT与其受体相互作用后的细胞效应很重要，包括p38丝裂原活化蛋白激酶（MAPK）的磷酸化。此外，使用预测工具的分析表明NPNT参与促进细胞活力。总之，我们的结果表明，NPNT通过其整合素结合基序，通过p38 MAPK的磷酸化促进细胞活力。
