Kurosawa Tamaki, Yamada Atsushi, Takami Masamichi, Suzuki Dai, Saito Yoshiro, Hiranuma Katsuhiro, Enomoto Takuya, Morimura Naoko, Yamamoto Matsuo, Iijima Takehiko, Shirota Tatsuo, Itabe Hiroyuki, Kamijo Ryutaro
Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan ; Division of Biological Chemistry, Department of Molecular Biology, Showa University School of Pharmacy, Shinagawa, Tokyo 142-8555, Japan.
Department of Biochemistry, School of Dentistry, Showa University, Shinagawa, Tokyo 142-8555, Japan.
FEBS Open Bio. 2015 Apr 8;5:303-7. doi: 10.1016/j.fob.2015.04.001. eCollection 2015.
Nephronectin (Npnt), also called POEM, is an extracellular matrix protein considered to play critical roles as an adhesion molecule in the development and functions of various tissues, such as the kidneys, liver, and bones. In the present study, we examined the molecular mechanism of Npnt gene expression and found that oncostatin M (OSM) strongly inhibited Npnt mRNA expression in MC3T3-E1 cells from a mouse osteoblastic cell line. OSM also induced a decrease in Npnt expression in both time- and dose-dependent manners via both the JAK/STAT and MAPK pathways. In addition, OSM-induced inhibition of osteoblast differentiation was recovered by over-expression of Npnt. These results suggest that OSM inhibits Npnt expression via the JAK/STAT and MAPK pathways, while down-regulation of Npnt by OSM influences inhibition of osteoblast differentiation.
肾连蛋白（Npnt），也称为POEM，是一种细胞外基质蛋白，被认为作为一种粘附分子在各种组织（如肾脏、肝脏和骨骼）的发育和功能中发挥关键作用。在本研究中，我们研究了Npnt基因表达的分子机制，发现抑瘤素M（OSM）强烈抑制来自小鼠成骨细胞系的MC3T3-E1细胞中Npnt mRNA的表达。OSM还通过JAK/STAT和MAPK途径以时间和剂量依赖性方式诱导Npnt表达下降。此外，通过Npnt的过表达可恢复OSM诱导的成骨细胞分化抑制。这些结果表明，OSM通过JAK/STAT和MAPK途径抑制Npnt表达，而OSM对Npnt的下调影响成骨细胞分化的抑制。
