Zayed Rania A, Omran Dalia, Mokhtar Doha A, Zakaria Zinab, Ezzat Sameera, Soliman Mohamed A, Mobarak Lamiaa, El-Sweesy Hossam, Emam Ghada
Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.
Department of Endemic Medicine and Hepato-gastroentrology, Faculty of Medicine, Cairo University, Cairo, Egypt.
Am J Trop Med Hyg. 2017 Mar;96(3):720-726. doi: 10.4269/ajtmh.16-0644. Epub 2017 Apr 6.
Toll-like receptors (TLRs) are recognized as fundamental contributors to the immune system function against infections. Hepatitis C virus (HCV) infection represents a global health problem especially in Egypt having the highest HCV prevalence worldwide where HCV infection is a continuing epidemic. The aim of the present study was to investigate the possible association between genetic variation in TLR-3 and TLR-9 and HCV infection and hepatic fibrosis in chronic HCV-positive Egyptian patients. The present study included 100 naïve chronic HCV-positive patients and 100 age- and sex-matched healthy controls. Genotyping of TLR-3 (_7 C/A [rs3775296]), TLR-3 (c.1377C/T [rs3775290]) and TLR-9 (1237T/C [rs5743836]) were done by polymerase chain reaction restriction fragment length polymorphism technique. Frequency of polymorphic genotypes in TLR-3 (_7 C/A), TLR-3 (c.1377C/T) and TLR-9 (1237T/C) were not significantly different between studied HCV-positive patients and controls with values 0.121, 0.112, and 0.683, respectively. TLR-3 c.1377 T-allele was associated with advanced stage of hepatic fibrosis ( = 0.003).
Toll样受体（TLRs）被认为是免疫系统抗感染功能的重要贡献者。丙型肝炎病毒（HCV）感染是一个全球性的健康问题，尤其是在埃及，该国的HCV患病率全球最高，HCV感染呈持续流行态势。本研究的目的是调查TLR-3和TLR-9基因变异与慢性HCV阳性埃及患者的HCV感染及肝纤维化之间的可能关联。本研究纳入了100例初治慢性HCV阳性患者和100例年龄及性别匹配的健康对照。采用聚合酶链反应-限制性片段长度多态性技术对TLR-3（_7 C/A [rs3775296]）、TLR-3（c.1377C/T [rs3775290]）和TLR-9（1237T/C [rs5743836]）进行基因分型。在研究的HCV阳性患者和对照中，TLR-3（_7 C/A）、TLR-3（c.1377C/T）和TLR-9（1237T/C）多态基因型的频率无显著差异，其P值分别为0.121、0.112和0.683。TLR-3 c.1377 T等位基因与肝纤维化晚期相关（P = 0.003）。
