Du Yuxuan, Li Shumin, Wang Xinyu, Liu Jialu, Gao Yan, Lv Weimiao, Liu Ping, Huang Haiyan, Luan Junwen, Zhang Leiliang
School of Clinical and Basic Medical Sciences & Institute of Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China.
School of Public Health, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, China.
Front Microbiol. 2023 Oct 5;14:1254805. doi: 10.3389/fmicb.2023.1254805. eCollection 2023.
The objective of this study is to investigate the association between toll-like receptor (TLR) 3/7 gene polymorphisms and the infection by hepatitis C virus (HCV).
PubMed, Embase, Web of Science, Scopus, CNKI, Wanfang Data, and SinoMed were searched to identify studies focusing on the association between the TLR3 rs3775290 or the TLR7 rs179008 single nucleotide polymorphisms (SNPs) and the HCV infection. All the related articles were collected from the inception of each database to 15 January 2023. Our meta-analysis was conducted using the allelic model, the dominant model, and the recessive model. Outcomes were presented by odds ratio (ORs) and 95% confidence interval (95%CI). The heterogeneity across studies was assessed by the I test. A subgroup analysis was performed to explore the source of heterogeneity. Funnel plots were drawn to assess the risk of publication bias. Review Manager 5.4 was used for statistical analysis.
Ten articles were finally included, among which six studies were analyzed for rs3775290 and five studies were analyzed for rs179008. Studies relating to rs3775290 included 801 patients and 1,045 controls, whereas studies relating to rs179008 included 924 patients and 784 controls. The results of the meta-analysis showed that there is no significant association between rs3775290 gene polymorphism and HCV infection (T vs. C: OR = 1.12, 95%CI 0.97-1.30; TT+CT vs. CC: OR = 1.20, 95%CI 0.73-1.96; TT vs. CT+CC: OR = 1.13, 95%CI 0.68-1.89). The recessive model showed that rs179008-T allele homozygotes had an 89% increased risk of infection by HCV compared with rs179008-A allele carriers (TT vs. AT+AA: OR = 1.89, 95%CI 1.13-3.16). The results of the subgroup analysis demonstrated that the characteristics of the control population may serve as an important source of heterogeneity. In the African populations, individuals with homozygous rs179008-T alleles had a higher risk of infection by HCV than rs179008-A allele carriers (OR = 2.14, 95%CI 1.18-3.87). We did not find that this difference existed in the European populations (OR = 1.24, 95%CI 0.43-3.56).
There is no significant association between rs3775290 single nucleotide polymorphism and the infection by HCV. Individuals with homozygous rs179008-T alleles have a higher risk of an infection by HCV than rs179008-A allele carriers, which is statistically significant in the African populations.
本研究旨在探讨Toll样受体（TLR）3/7基因多态性与丙型肝炎病毒（HCV）感染之间的关联。
检索PubMed、Embase、Web of Science、Scopus、中国知网、万方数据和中国生物医学文献服务系统，以识别关注TLR3 rs3775290或TLR7 rs179008单核苷酸多态性（SNP）与HCV感染之间关联的研究。从每个数据库建库至2023年1月15日收集所有相关文章。我们使用等位基因模型、显性模型和隐性模型进行荟萃分析。结果以比值比（OR）和95%置信区间（95%CI）表示。通过I²检验评估研究间的异质性。进行亚组分析以探索异质性来源。绘制漏斗图以评估发表偏倚风险。使用Review Manager 5.4进行统计分析。
最终纳入10篇文章，其中6项研究分析rs3775290，5项研究分析rs179008。与rs3775290相关的研究纳入801例患者和1045例对照，而与rs179008相关的研究纳入924例患者和784例对照。荟萃分析结果显示，rs3775290基因多态性与HCV感染之间无显著关联（T与C：OR = 1.12，95%CI 0.97 - 1.30；TT + CT与CC：OR = 1.20，95%CI 0.73 - 1.96；TT与CT + CC：OR = 1.13，95%CI 0.68 - 1.89）。隐性模型显示，与rs179008 - A等位基因携带者相比，rs179008 - T等位基因纯合子感染HCV的风险增加89%（TT与AT + AA：OR = 1.89，95%CI 1.13 - 3.16）。亚组分析结果表明，对照人群的特征可能是异质性的重要来源。在非洲人群中，rs179008 - T等位基因纯合个体感染HCV的风险高于rs179008 - A等位基因携带者（OR = 2.14，95%CI 1.18 - 3.87）。我们未发现欧洲人群存在这种差异（OR = 1.24，95%CI 0.43 - 3.56）。
rs37多态性与HCV感染之间无显著关联。rs179008 - T等位基因纯合个体感染HCV的风险高于rs179008 - A等位基因携带者，这在非洲人群中具有统计学意义。
