Marumo K
Department of Orthopaedic Surgery, Jikei University, School of Medicine, Tokyo, Japan.
Nihon Seikeigeka Gakkai Zasshi. 1989 Aug;63(8):852-9.
Polyphenolic or adhesive protein (PPP, Mr = 125,000) from the marine mussel Mytilus edulis consists largely of tandemly repeated decapeptide sequence, ala-lys-pro/hyp-ser-tyr/dopa-hyp-hyp-thr-dopa-lys. Because of our interest in better understanding of the mechanism of under water adhesion by this protein, we have attempted to ascertain the adhesiveness of PPP to a cultured chondrocyte. About 90% of the chondrocyte were rapidly attached to the dish coated by PPP within 60 minutes. A protein with 216 amino acids and molecular weight of 24,000 has 20 repeats of decapeptide sequence (20 mer), which designed as a synthetic analogue of proto-PPP. Nearly 44% of tyrosyl group in the 20 mer were converted to dopa which is thought to play an essential role in the adhesive and cohesive properties of the protein by mushroom tyrosinase. The dopa conversion ratios of tyr-5 (-ser-tyr-pro-) and tyr-9 (-thr-tyr-lys) in the decapeptide sequence of the 20 mer were very similar to those of PPP. These findings may contribute to design a new type of adhesives for living tissues.
来自海洋贻贝紫贻贝的多酚或粘附蛋白（PPP，分子量 = 125,000）主要由串联重复的十肽序列ala-lys-pro/hyp-ser-tyr/dopa-hyp-hyp-thr-dopa-lys组成。由于我们有兴趣更好地了解这种蛋白质在水下的粘附机制，我们试图确定PPP对培养的软骨细胞的粘附性。大约90%的软骨细胞在60分钟内迅速附着在涂有PPP的培养皿上。一种含有216个氨基酸、分子量为24,000的蛋白质具有20个十肽序列重复（20聚体），被设计为原始PPP的合成类似物。20聚体中近44%的酪氨酸基团被转化为多巴，多巴被认为在该蛋白质的粘附和内聚性能中起重要作用，这是通过蘑菇酪氨酸酶实现的。20聚体十肽序列中tyr-5（-ser-tyr-pro-）和tyr-9（-thr-tyr-lys）的多巴转化率与PPP的非常相似。这些发现可能有助于设计一种新型的生物组织粘合剂。
