Waite J H, Housley T J, Tanzer M L
Biochemistry. 1985 Sep 10;24(19):5010-4. doi: 10.1021/bi00340a008.
The adhesive protein from Mytilus edulis contains 75-80 closely related, repeated peptide sequences in its primary structure. These peptides can be resolved following digestion with trypsin by reversed-phase high-pressure liquid chromatography. The most frequently repeated sequence is the decapeptide Ala-Lys-Pro-Ser-Tyr-Hyp-Hyp-Thr-Dopa-Lys (peptide E). Variations of this occur in peptides B with Hyp-3 and Dopa-5, C with Dopa-5, and D with Hyp-3, respectively. Lesser amounts of hexapeptides (A and B') that are lacking residues 4-7 also occur. Peptide A has the sequence Ala-Lys-Pro-Thr-Dopa-Lys, whereas B' contains Tyr instead of Dopa. 4-Hydroxyproline occurs at positions 3 and 7 and occasionally at position 6 of the decapeptide; 3-hydroxyproline occurs only at position 6. Adhesiveness of the protein may be related to the repetition of Dopa residues, the catecholic moiety of which has strong hydrogen-bonding and metal-liganding capabilities.
紫贻贝的粘附蛋白在其一级结构中含有75 - 80个紧密相关的重复肽序列。用胰蛋白酶消化后，这些肽可通过反相高压液相色谱法分离。最常见的重复序列是十肽Ala-Lys-Pro-Ser-Tyr-Hyp-Hyp-Thr-Dopa-Lys（肽E）。在肽B中，该序列的第3位为Hyp，第5位为Dopa；在肽C中，第5位为Dopa；在肽D中，第3位为Hyp，分别形成了该序列的变体。还存在少量缺少4 - 7位残基的六肽（A和B'）。肽A的序列为Ala-Lys-Pro-Thr-Dopa-Lys，而B'中含有Tyr而非Dopa。十肽的第3位和第7位出现4 - 羟基脯氨酸，偶尔第6位也会出现；3 - 羟基脯氨酸仅出现在第6位。该蛋白的粘附性可能与Dopa残基的重复有关，其儿茶酚部分具有很强的氢键形成能力和金属配位能力。
