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加州贻贝(Mytilus californianus Conrad)的贻贝胶:一项比较研究。

Mussel glue from Mytilus californianus Conrad: a comparative study.

作者信息

Waite J H

出版信息

J Comp Physiol B. 1986;156(4):491-6. doi: 10.1007/BF00691034.

DOI:10.1007/BF00691034
PMID:3734192
Abstract

Marine mussels secrete a byssus in order to attach to solid surfaces in the sea. The polyphenolic protein is the "glue" in the adhesive plaques of the byssus. In Mytilus californianus, the polyphenolic protein has an apparent molecular weight of 85,000 +/- 5,000 and is rich in the amino acids lysine, 3,4-dihydroxyphenylalanine, serine, threonine, and hydroxyproline. In composition it resembles the polyphenolic protein of M. edulis (Mr = 125,000), although the M. edulis protein contains significantly less isoleucine and more alanine. Tryptic digestion of M. californianus polyphenolic protein revealed two types of repeating decapeptides (1) (Ser/Thr)-Thr-(Tyr/Dopa)-Hyp-Hyp-Thr-Dopa-Lys-Hyp-Lys and (2) Ile-(Thr/Ser)-(Tyr/Dopa)-Hyp-Hyp-Thr-Dopa-Lys-Hyp-Lys. Residues 2 to 8 are identical with residues 4-10 in M. edulis decapeptides.

摘要

海洋贻贝分泌足丝以便附着在海洋中的固体表面。多酚蛋白是足丝附着斑中的“胶水”。在加州贻贝中,多酚蛋白的表观分子量为85,000±5,000,富含赖氨酸、3,4-二羟基苯丙氨酸、丝氨酸、苏氨酸和羟脯氨酸。在组成上,它类似于紫贻贝的多酚蛋白(Mr = 125,000),尽管紫贻贝蛋白中的异亮氨酸含量明显较少,丙氨酸含量较多。对加州贻贝多酚蛋白进行胰蛋白酶消化后,发现了两种重复的十肽:(1)(丝氨酸/苏氨酸)-苏氨酸-(酪氨酸/多巴)-羟脯氨酸-羟脯氨酸-苏氨酸-多巴-赖氨酸-羟脯氨酸-赖氨酸和(2)异亮氨酸-(苏氨酸/丝氨酸)-(酪氨酸/多巴)-羟脯氨酸-羟脯氨酸-苏氨酸-多巴-赖氨酸-羟脯氨酸-赖氨酸。第2至8位残基与紫贻贝十肽中的第4至10位残基相同。

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本文引用的文献

1
Polyphenolic Substance of Mytilus edulis: Novel Adhesive Containing L-Dopa and Hydroxyproline.贻贝的多酚类物质:含L-多巴和羟脯氨酸的新型粘合剂。
Science. 1981 May 29;212(4498):1038-40. doi: 10.1126/science.212.4498.1038.
2
Chemical structure of rat liver cytochrome b5. Isolation of peptides by high-pressure liquid chromatography.大鼠肝脏细胞色素b5的化学结构。通过高压液相色谱法分离肽段。
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Biochemistry. 2009 Mar 31;48(12):2752-9. doi: 10.1021/bi900018m.
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Adhesive protein cDNA sequence of the mussel Mytilus coruscus and its evolutionary implications.波纹巴非蛤贻贝黏附蛋白cDNA序列及其进化意义。
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8
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J Comp Physiol B. 1989;159(5):517-25. doi: 10.1007/BF00694376.
Methods Enzymol. 1984;107:397-413. doi: 10.1016/0076-6879(84)07028-2.
4
Porosity and specific surface of bone.
Crit Rev Biomed Eng. 1984;10(3):179-222.
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Evidence for a repeating 3,4-dihydroxyphenylalanine- and hydroxyproline-containing decapeptide in the adhesive protein of the mussel, Mytilus edulis L.贻贝(紫贻贝)黏附蛋白中含3,4-二羟基苯丙氨酸和羟脯氨酸的重复十肽的证据
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High resolution acrylamide gel electrophoresis of histones.组蛋白的高分辨率丙烯酰胺凝胶电泳
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The structure of telomycin.端粒霉素的结构。
J Am Chem Soc. 1968 Jan 17;90(2):462-70. doi: 10.1021/ja01004a043.
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