从噬菌体展示中筛选出的颗粒物结合肽的表征

Characterization of particulate matter binding peptides screened from phage display.

作者信息

Liang Alvin Aw Wei, Tanaka Masayoshi, Okochi Mina

机构信息

Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8552, Japan.

Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8552, Japan; Office for the Impulsing Paradigm Change Through Disruptive Technologies Program (ImPACT), Japan Science and Technology Agency (JST), Tokyo Headquarters 2 K's Gobancho 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.

出版信息

J Biosci Bioeng. 2017 May;123(5):621-624. doi: 10.1016/j.jbiosc.2016.12.014. Epub 2017 Jan 13.

DOI:10.1016/j.jbiosc.2016.12.014

PMID:28094103

Abstract

Particulate matter (PM), especially particulates with diameters of less than 2.5 μm, can penetrate the alveolar region and increase the risk of respiratory diseases. This has stimulated research efforts to develop detection methods so that counter measures can be taken. In this study, four PM binding peptides were obtained by phage display and binding characteristics of these peptides were investigated using the peptide array. The strongest binding peptide, WQDFGAVRSTRS, displayed a binding property, measured in terms of spot intensity, 11.4 times higher than that of the negative control, AAAAA. Inductively coupled plasma mass spectrometry (ICPMS) analysis of the transition metal compounds in the PM bound to the peptide spots was performed, and two peptides showed higher binding towards Cu and Zn compounds in PM. These results suggest that the screened peptides could serve as an indicator of transition metal compounds, which are related to adverse health effects, contained in PM.

摘要

颗粒物（PM），尤其是直径小于2.5微米的颗粒物，能够穿透肺泡区域并增加患呼吸道疾病的风险。这激发了人们研发检测方法的研究工作，以便能够采取应对措施。在本研究中，通过噬菌体展示获得了四种PM结合肽，并使用肽阵列研究了这些肽的结合特性。结合能力最强的肽WQDFGAVRSTRS，以斑点强度衡量，其结合特性比阴性对照AAAAA高11.4倍。对与肽斑点结合的PM中的过渡金属化合物进行了电感耦合等离子体质谱（ICPMS）分析，有两种肽对PM中的铜和锌化合物表现出更高的结合能力。这些结果表明，筛选出的肽可作为PM中与不良健康影响相关的过渡金属化合物的指标。