Department of Chemical Science and Engineering, Tokyo Institute of Technology, 2-12-1, O-okayama, Meguro-ku, Tokyo 152-8552, Japan.
School of Science; Tokyo Institute of Technology, 2-12-1, O-okayama, Meguro-ku, Tokyo 152-8552, Japan.
Int J Mol Sci. 2020 Mar 30;21(7):2377. doi: 10.3390/ijms21072377.
The use of biomolecules in nanomaterial synthesis has received increasing attention, because they can function as a medium to produce inorganic materials in ambient conditions. Short peptides are putative ligands that interact with metallic surfaces, as they have the potential to control the synthesis of nanoscale materials. Silver nanoparticle (AgNP) mineralization using peptides has been investigated; however, further comprehensive analysis must be carried out, because the design of peptide mediated-AgNP properties is still highly challenging. Herein, we employed an array comprising 200 spot synthesis-based peptides, which were previously isolated as gold nanoparticle (AuNP)-binding and/or mineralization peptides, and the AgNP mineralization activity of each peptide was broadly evaluated. Among 10 peptides showing the highest AgNP-synthesis activity (TOP10), nine showed the presence of EE and E[X]E (E: glutamic acid, and X: any amino acid), whereas none of these motifs were found in the WORST25 (25 peptides showing the lowest AgNP synthesis activity) peptides. The size and morphology of the particles synthesized by TOP3 peptides were dependent on their sequences. These results suggested not only that array-based techniques are effective for the peptide screening of AgNP mineralization, but also that AgNP mineralization regulated by peptides has the potential for the synthesis of AgNPs, with controlled morphology in environmentally friendly conditions.
生物分子在纳米材料合成中的应用受到了越来越多的关注，因为它们可以作为在环境条件下生产无机材料的媒介。短肽是与金属表面相互作用的假定配体，因为它们有可能控制纳米级材料的合成。已经研究了使用肽来进行银纳米颗粒（AgNP）矿化，但是必须进行更全面的分析，因为设计由肽介导的 AgNP 性质仍然极具挑战性。在这里，我们使用了包含 200 个斑点合成基肽的阵列，这些肽先前被分离为金纳米颗粒（AuNP）结合和/或矿化肽，并且广泛评估了每种肽的 AgNP 矿化活性。在显示出最高 AgNP 合成活性的 10 种肽（TOP10）中，有 9 种含有 EE 和 E[X]E（E：谷氨酸，X：任何氨基酸），而在显示出最低 AgNP 合成活性的 25 种肽（WORST25）中则没有这些基序。TOP3 肽合成的颗粒的大小和形态取决于它们的序列。这些结果不仅表明基于阵列的技术对于 AgNP 矿化的肽筛选是有效的，而且还表明由肽调节的 AgNP 矿化有可能在环保条件下合成具有可控形态的 AgNPs。
