Harbuz M S, Lightman S L
Medical Unit, Charing Cross and Westminster Medical School, Westminster Hospital, London.
J Endocrinol. 1989 Sep;122(3):705-11. doi: 10.1677/joe.0.1220705.
In-situ hybridization histochemistry was used to measure corticotrophin-releasing factor mRNA and proenkephalin A mRNA in the paraventricular nucleus (PVN), and pro-opiomelanocortin (POMC) mRNA in the anterior pituitary of the rat. Levels of message were determined at 1, 2, 4 and 8 h after exposure to a variety of physical and psychological stresses. Corticotrophin-releasing factor mRNA in the PVN and POMC mRNA in the anterior pituitary increased in response to i.p. hypertonic saline, restraint and swim stress but not to cold stress. Proenkephalin A mRNA was raised only in response to the physical stress of i.p. injection of hypertonic saline. These results suggest that different afferent pathways and hypothalamic neurotransmitters may be involved in mediating the hypothalamic response to different physical and psychological stresses.
采用原位杂交组织化学法检测大鼠室旁核（PVN）中促肾上腺皮质激素释放因子mRNA和前脑啡肽原A mRNA，以及垂体前叶中阿黑皮素原（POMC）mRNA的表达。在暴露于多种生理和心理应激源后1、2、4和8小时测定信息水平。室旁核中的促肾上腺皮质激素释放因子mRNA和垂体前叶中的POMC mRNA在腹腔注射高渗盐水、束缚和游泳应激后增加，但对冷应激无反应。前脑啡肽原A mRNA仅在腹腔注射高渗盐水的生理应激后升高。这些结果表明，不同的传入途径和下丘脑神经递质可能参与介导下丘脑对不同生理和心理应激的反应。
