白藜芦醇通过保护纤维蛋白原对血小板聚集的影响。

Effect of resveratrol on platelet aggregation by fibrinogen protection.

作者信息

Bonechi Claudia, Lamponi Stefania, Donati Alessandro, Tamasi Gabriella, Consumi Marco, Leone Gemma, Rossi Claudio, Magnani Agnese

机构信息

Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; CSGI-Research Center for Colloids and Nanoscience, Via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy.

Department of Biotechnologies, Chemistry, and Pharmacy, Via Aldo Moro 2, 53100 Siena, Italy; INSTM-National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti 9, 50121 Firenze, Italy.

出版信息

Biophys Chem. 2017 Mar;222:41-48. doi: 10.1016/j.bpc.2016.12.004. Epub 2017 Jan 2.

DOI:10.1016/j.bpc.2016.12.004

PMID:28095333

Abstract

The effect of resveratrol (RSV) in inhibiting platelet adhesion and aggregation, as well as fibrinogen (FBG) conformational changes promoted by epinephrine (EP), were studied, by using complementary experimental techniques. NMR and IR spectroscopies were used to investigate possible protective effects by RSV towards FBG, in presence of EP. The protective effect of RSV towards FBG was highlighted by spin nuclear relaxation experiments that were interpreted for determining the thermodynamic equilibrium constants of FBG-EP interaction, and by infrared measurements, that showed EP-induced conformational changes of FBG. The ability of RSV in inhibiting platelet adhesion and aggregation promoted by EP was evaluated by scanning electron microscopy (SEM), measuring the platelet adhesion and aggregation degree, in comparison to data obtained for platelet aggregation in platelet rich plasma (PRP). The experimental combined approach pointed out that RSV is able to protect both FBG and platelets from the denaturant and aggregating action of EP at stress level concentration.

摘要

采用互补实验技术研究了白藜芦醇（RSV）抑制血小板黏附和聚集的作用，以及肾上腺素（EP）促进的纤维蛋白原（FBG）构象变化。利用核磁共振（NMR）和红外光谱（IR）研究了在EP存在下RSV对FBG可能的保护作用。通过自旋核弛豫实验（用于确定FBG-EP相互作用的热力学平衡常数）和红外测量（显示EP诱导的FBG构象变化）突出了RSV对FBG的保护作用。通过扫描电子显微镜（SEM）评估RSV抑制EP促进的血小板黏附和聚集的能力，测量血小板黏附和聚集程度，并与富血小板血浆（PRP）中血小板聚集的数据进行比较。实验综合方法指出，在应激水平浓度下，RSV能够保护FBG和血小板免受EP的变性和聚集作用。