Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China; WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious Diseases, Guangzhou, China.
Guangdong Provincial Center for Disease Control and Prevention, Guangzhou, China; WHO Collaborating Centre for Surveillance, Research and Training of Emerging Infectious Diseases, Guangzhou, China.
J Infect. 2017 Oct;75(4):356-367. doi: 10.1016/j.jinf.2017.07.001. Epub 2017 Jul 14.
Fast expansion and linkage to microcephaly and Guillain Barre syndrome have made Zika virus (ZIKV) track attention of global health authority concerns. The epidemiology, virological characteristics and genetic evolution of introduced ZIKV to Guangdong, China, were investigated.
Analyses of the epidemiological characteristics and genetic diversity of ZIKV isolates were performed.
A total of twenty-eight confirmed ZIKV infection cases were imported into China in 2016, of which 19 were imported into Guangdong, China from Venezuela (16), the Samoa Islands (1), Suriname (1) and Guatemala (1). Serial sampling studies of the cases indicated longer shedding times of ZIKV particles from urine and saliva samples than from serum and conjunctiva swab samples. Seven ZIKV strains were successfully isolated from serum, urine and conjunctiva swab samples using cell culture and neonatal mouse injection methods. Genomic analysis indicated that all viruses belonged to the Asian lineage but had different evolutionary transmission routes with different geographic origins. The molecular clock phylogenetic analysis of the ZIKV genomes indicated independent local transmission that appeared to have been previously established in Venezuela and Samoa. Additionally, we found 7 unique non-synonymous mutations in the genomes of ZIKV that were imported to China. The mutations may indicate that ZIKV has undergone independent evolutionary history not caused by sudden adaptation to Chinese hosts.
The increasing number of ex-patriot Chinese returning from ZIKV hyper-endemic areas to Guangdong combined with the presence of a variety of Aedes species indicate the potential for autochthonous transmission of ZIKV in Guangdong.
寨卡病毒（ZIKV）的快速传播及其与小头症和格林-巴利综合征的关联引起了全球卫生机构的关注。本研究旨在调查引入中国广东的寨卡病毒的流行病学、病毒学特征和遗传进化。
对寨卡病毒分离株的流行病学特征和遗传多样性进行分析。
2016 年共有 28 例确诊的寨卡病毒感染病例输入中国，其中 19 例从委内瑞拉（16 例）、萨摩亚群岛（1 例）、苏里南（1 例）和危地马拉（1 例）输入广东。对病例的系列采样研究表明，尿液和唾液样本中寨卡病毒颗粒的排出时间长于血清和结膜拭子样本。采用细胞培养和新生鼠注射法从血清、尿液和结膜拭子样本中成功分离出 7 株寨卡病毒。基因组分析表明，所有病毒均属于亚洲谱系，但具有不同的进化传播途径，起源于不同的地理区域。寨卡病毒基因组的分子钟系统发育分析表明，寨卡病毒在委内瑞拉和萨摩亚已经存在独立的本地传播，此外，我们在中国输入的寨卡病毒基因组中发现了 7 个独特的非同义突变。这些突变可能表明寨卡病毒经历了独立的进化历史，而不是由于突然适应中国宿主而产生的。
越来越多的从寨卡病毒高流行地区返回广东的侨民，加上存在多种伊蚊种类，表明寨卡病毒在广东可能存在本地传播。
