Li Wen-Chao, Wang Kai, Zhang Wei, Wu Jingjing, Gu You-Fang, Zhang Xi-Chen
College of Animal Science, Anhui Science and Technology University, Fengyang, 233100, P. R. China.
College of Veterinary Medicine, Jilin University, Changchun, 130062, P. R. China.
Korean J Parasitol. 2016 Dec;54(6):703-710. doi: 10.3347/kjp.2016.54.6.703. Epub 2016 Dec 31.
The trichomonad species and were recently detected in the feces of dogs with diarrhea. However, little information is available on the prevalence and pathogenicity of these parasites in the canine population. Therefore, the aim of this study was to determine the prevalence and molecular characterization of trichomonads infecting pet dogs in Anhui and Zhejiang provinces, east China. In total, 315 pet dogs, with or without diarrhea, from 7 pet hospitals were included in this epidemiological survey. Microscopy and PCR detected in 19.7% (62/315) and 31.4% (99/315) of fecal samples, respectively. infection was detected in 0% (0/315) of samples with microscopy and in 0.6% (2/315) with PCR. The prevalence of was significantly higher in young dogs (≤12 months) than in adult dogs (>12 months), and was significantly higher in diarrheic dogs (50.6%) than in non-diarrheic dogs (24.3%; <0.05). Infection with did not correlate with any risk factors evaluated in this study. A sequence analysis of the PCR products showed minor allelic variations between our sequences and those of strains from other hosts in different parts of the world. Type CC1 was the most common strain in dogs in east China. The internal transcribed spacer 1 (ITS1)-5.8S rRNA gene sequences from the 2 isolates detected in this study displayed 100% identity and were homologous to the sequences of other strains isolated from domestic cats in other countries.
最近在腹泻犬的粪便中检测到了毛滴虫属物种和。然而，关于这些寄生虫在犬类群体中的流行情况和致病性的信息却很少。因此，本研究的目的是确定感染中国东部安徽省和浙江省宠物犬的毛滴虫的流行情况和分子特征。在这项流行病学调查中，总共纳入了来自7家宠物医院的315只宠物犬，无论有无腹泻症状。显微镜检查和聚合酶链反应（PCR）分别在19.7%（62/315）和31.4%（99/315）的粪便样本中检测到。显微镜检查在0%（0/315）的样本中检测到感染，PCR检测在0.6%（2/315）的样本中检测到感染。在幼犬（≤12个月）中的流行率显著高于成年犬（>12个月），在腹泻犬中的流行率（50.6%）显著高于非腹泻犬（24.3%；<0.05）。感染与本研究中评估的任何风险因素均无关联。对PCR产物的序列分析表明，我们的序列与世界不同地区其他宿主的菌株序列之间存在微小的等位基因变异。CC1型是中国东部犬类中最常见的菌株。本研究中检测到的2株分离株的内部转录间隔区1（ITS1）-5.8S rRNA基因序列显示出100%的同一性，并且与从其他国家的家猫分离出的其他菌株序列同源。
