Sousa Fernanda, Sanavio Barbara, Saccani Alessandra, Tang Yun, Zucca Ileana, Carney Tamara M, Mastropietro Alfonso, Jacob Silva Paulo H, Carney Randy P, Schenk Kurt, Omrani Arash O, Huang Ping, Yang Lin, Rønnow Henrik M, Stellacci Francesco, Krol Silke
Nanomedicine Laboratory, Fondazione IRCCS Istituto Neurologico Carlo Besta , AMADEOLAB, Via G.A. Amadeo 42, 20133 Milan, Italy.
IFOM The FIRC Institute of Molecular Oncology , Via Adamello 16, 20139 Milan, Italy.
Bioconjug Chem. 2017 Jan 18;28(1):161-170. doi: 10.1021/acs.bioconjchem.6b00577. Epub 2016 Nov 28.
Nanoparticle-based magnetic resonance imaging T negative agents are of great interest, and much effort is devoted to increasing cell-loading capability while maintaining low cytotoxicity. Herein, two classes of mixed-ligand protected magnetic-responsive, bimetallic gold/iron nanoparticles (Au/Fe NPs) synthesized by a two-step method are presented. Their structure, surface composition, and magnetic properties are characterized. The two classes of sulfonated Au/Fe NPs, with an average diameter of 4 nm, have an average atomic ratio of Au to Fe equal to 7 or 8, which enables the Au/Fe NPs to be superparamagnetic with a blocking temperature of 56 K and 96 K. Furthermore, preliminary cellular studies reveal that both Au/Fe NPs show very limited toxicity. MRI phantom experiments show that r/r ratio of Au/Fe NPs is as high as 670, leading to a 66% reduction in T relaxation time. These nanoparticles provide great versatility and potential for nanoparticle-based diagnostics and therapeutic applications and as imaging contrast agents.
基于纳米颗粒的磁共振成像T阴性造影剂备受关注，人们致力于在保持低细胞毒性的同时提高细胞负载能力。在此，介绍了通过两步法合成的两类混合配体保护的磁响应双金属金/铁纳米颗粒（Au/Fe NPs）。对其结构、表面组成和磁性进行了表征。这两类磺化Au/Fe NPs的平均直径为4 nm，金与铁的平均原子比为7或8，这使得Au/Fe NPs具有超顺磁性，其阻塞温度分别为56 K和96 K。此外，初步细胞研究表明，两种Au/Fe NPs均显示出非常有限的毒性。MRI体模实验表明，Au/Fe NPs的r/r比率高达670，导致T弛豫时间减少66%。这些纳米颗粒为基于纳米颗粒的诊断和治疗应用以及作为成像造影剂提供了极大的通用性和潜力。
