Bulman Zackery P, Ly Neang S, Lenhard Justin R, Holden Patricia N, Bulitta Jürgen B, Tsuji Brian T
Laboratory for Antimicrobial Pharmacodynamics, University at Buffalo School of Pharmacy and Pharmaceutical Sciences, Buffalo, New York, USA.
New York State Center of Excellence in Life Sciences and Bioinformatics, Buffalo, New York, USA.
Antimicrob Agents Chemother. 2017 Mar 24;61(4). doi: 10.1128/AAC.00096-16. Print 2017 Apr.
The impact of quorum sensing on polymyxin and azithromycin pharmacodynamics was assessed in PAO1 and an isogenic / double knockout. For polymyxin B, greater killing against the / knockout than against PAO1 was observed at 10 CFU/ml (polymyxin B half-maximal effective concentration [EC], 5.61 versus 12.5 mg/liter, respectively; < 0.005). Polymyxin B combined with azithromycin (256 mg/liter) was synergistic against each strain, significantly reducing the respective polymyxin B EC compared to those with monotherapy ( < 0.005), and is a promising strategy by which to combat .
在铜绿假单胞菌PAO1及其同基因/双敲除菌株中评估了群体感应对多粘菌素和阿奇霉素药效学的影响。对于多粘菌素B，在10 CFU/ml时观察到对双敲除菌株的杀灭作用比对PAO1更强（多粘菌素B的半数有效浓度[EC]分别为5.61和12.5 mg/升；P<0.005）。多粘菌素B与阿奇霉素（256 mg/升）联合使用对每种菌株都具有协同作用，与单药治疗相比，显著降低了各自的多粘菌素B的EC（P<0.005），这是一种对抗[相关疾病]的有前景的策略。
