1,3-二氨基丙烷通过与群体感应系统的相互作用抑制 生物膜的形成。
Inhibitory effects of 1,3-diaminopropane on the biofilm formation of via interaction with quorum sensing system.
机构信息
Department of Laboratory Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021;46(9):942-948. doi: 10.11817/j.issn.1672-7347.2021.200810.
OBJECTIVES
To study the inhibitory effects of 1,3-diaminopropane on the biofilm formation of and the underlying mechanisms.
METHODS
The experiment was divided into an experimental group and a control group. Crystal violet staining was used to examine the inhibitory effects of 1,3-diaminopropane on the biofilm formation of , and the biofilm formation was compared between the 2 groups.Initial adherence inhibition assay and swimming plate assay were used to determine the inhibitory effects of 1,3-diaminopropane on the initial adherence and swimming motility of ,and the quantification of adhered cells and swimming diameter were compared between the 2 groups. Meanwhile, Western blotting was used to detect the Flagellin production of ; real-time RT-PCR was used to detect the quorum sensing system relative genes and flagellum regulative related genes expression in the 2 groups. Finally, molecular docking assay was used to calculate the interaction between 1,3-diaminopropane and LasI.
RESULTS
Compared with the control group, the biofilm formation of was significantly inhibited in the experimental group in a dose-dependent manner (=6.07, <0.01).Compared with the control group, the initial adherence of could significantly inhibit from (0.890±0.389)×10 to (0.245±0.076)×10 CFU/mL (=3.257, <0.05) in the experimental group (2.0 mmol/L).Compared with the control group, the swimming motility of flagellar mediation could also inhibit in the experimental group (2.0 mmol/L). The swimming motility diameter was from (1.840±0.144) to (0.756±0.222) cm (=7.099, <0.01). Compared with the control group, the Flagellin production was inhibited in the experimental group. Finally, the molecular docking assay showed that the potential target of 1,3-diaminopropane was LasI.
CONCLUSIONS
1,3-diaminopropane can significantly inhibit the biofilm formation of , which potentially targets LasI protein.
目的
研究 1,3-二氨基丙烷对 生物膜形成的抑制作用及其机制。
方法
实验分为实验组和对照组。结晶紫染色法检测 1,3-二氨基丙烷对 生物膜形成的抑制作用，比较两组生物膜形成情况。初始黏附抑制试验和泳动平板试验检测 1,3-二氨基丙烷对 初始黏附和泳动运动能力的抑制作用，比较两组黏附细胞数量和泳动直径。同时，用 Western blot 检测 Flagellin 的产生；用实时 RT-PCR 检测两组群体感应系统相对基因和鞭毛调节相关基因的表达。最后，用分子对接试验计算 1,3-二氨基丙烷与 LasI 的相互作用。
结果
与对照组相比，实验组生物膜形成呈剂量依赖性显著抑制（=6.07，<0.01）。与对照组相比，实验组 1,3-二氨基丙烷（2.0 mmol/L）能显著抑制初始黏附，黏附细胞从（0.890±0.389）×10 到（0.245±0.076）×10 CFU/mL（=3.257，<0.05）。与对照组相比，实验组也能抑制鞭毛介导的运动能力。泳动直径从（1.840±0.144）到（0.756±0.222）cm（=7.099，<0.01）。与对照组相比，实验组 Flagellin 的产生受到抑制。最后，分子对接试验表明 1,3-二氨基丙烷的潜在靶点是 LasI。
结论
1,3-二氨基丙烷能显著抑制 生物膜形成，其潜在靶点是 LasI 蛋白。
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