Department of Laboratory Medicine, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2021;46(9):942-948. doi: 10.11817/j.issn.1672-7347.2021.200810.
To study the inhibitory effects of 1,3-diaminopropane on the biofilm formation of and the underlying mechanisms.
The experiment was divided into an experimental group and a control group. Crystal violet staining was used to examine the inhibitory effects of 1,3-diaminopropane on the biofilm formation of , and the biofilm formation was compared between the 2 groups.Initial adherence inhibition assay and swimming plate assay were used to determine the inhibitory effects of 1,3-diaminopropane on the initial adherence and swimming motility of ,and the quantification of adhered cells and swimming diameter were compared between the 2 groups. Meanwhile, Western blotting was used to detect the Flagellin production of ; real-time RT-PCR was used to detect the quorum sensing system relative genes and flagellum regulative related genes expression in the 2 groups. Finally, molecular docking assay was used to calculate the interaction between 1,3-diaminopropane and LasI.
Compared with the control group, the biofilm formation of was significantly inhibited in the experimental group in a dose-dependent manner (=6.07, <0.01).Compared with the control group, the initial adherence of could significantly inhibit from (0.890±0.389)×10 to (0.245±0.076)×10 CFU/mL (=3.257, <0.05) in the experimental group (2.0 mmol/L).Compared with the control group, the swimming motility of flagellar mediation could also inhibit in the experimental group (2.0 mmol/L). The swimming motility diameter was from (1.840±0.144) to (0.756±0.222) cm (=7.099, <0.01). Compared with the control group, the Flagellin production was inhibited in the experimental group. Finally, the molecular docking assay showed that the potential target of 1,3-diaminopropane was LasI.
1,3-diaminopropane can significantly inhibit the biofilm formation of , which potentially targets LasI protein.
研究 1,3-二氨基丙烷对 生物膜形成的抑制作用及其机制。
实验分为实验组和对照组。结晶紫染色法检测 1,3-二氨基丙烷对 生物膜形成的抑制作用，比较两组生物膜形成情况。初始黏附抑制试验和泳动平板试验检测 1,3-二氨基丙烷对 初始黏附和泳动运动能力的抑制作用，比较两组黏附细胞数量和泳动直径。同时，用 Western blot 检测 Flagellin 的产生；用实时 RT-PCR 检测两组群体感应系统相对基因和鞭毛调节相关基因的表达。最后，用分子对接试验计算 1,3-二氨基丙烷与 LasI 的相互作用。
与对照组相比，实验组生物膜形成呈剂量依赖性显著抑制（=6.07，<0.01）。与对照组相比，实验组 1,3-二氨基丙烷（2.0 mmol/L）能显著抑制初始黏附，黏附细胞从（0.890±0.389）×10 到（0.245±0.076）×10 CFU/mL（=3.257，<0.05）。与对照组相比，实验组也能抑制鞭毛介导的运动能力。泳动直径从（1.840±0.144）到（0.756±0.222）cm（=7.099，<0.01）。与对照组相比，实验组 Flagellin 的产生受到抑制。最后，分子对接试验表明 1,3-二氨基丙烷的潜在靶点是 LasI。
1,3-二氨基丙烷能显著抑制 生物膜形成，其潜在靶点是 LasI 蛋白。
