Sokurenko Yulia, Ulyanova Vera, Zelenikhin Pavel, Kolpakov Alexey, Blokhin Dmitriy, Müller Dieter, Klochkov Vladimir, Ilinskaya Olga
Institute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya Str. 18, Kazan 420008, Russia.
Institute of Physics, Kazan Federal University, Kremlevskaya Str. 16a, Kazan 420008, Russia.
Bioinorg Chem Appl. 2016;2016:4121960. doi: 10.1155/2016/4121960. Epub 2016 Dec 21.
Extracellular enzymes of intestinal microbiota are the key agents that affect functional activity of the body as they directly interact with epithelial and immune cells. Several species of the genus, like , a common producer of extracellular RNase binase, can populate the intestinal microbiome as a colonizing organism. Without involving metal ions as cofactors, binase depolymerizes RNA by cleaving the 3',5'-phosphodiester bond and generates 2',3'-cyclic guanosine phosphates in the first stage of a catalytic reaction. Maintained in the reaction mixture for more than one hour, such messengers can affect the human intestinal microflora and the human body. In the present study, we found that the rate of 2',3'-cGMP was growing in the presence of transition metals that stabilized the RNA structure. At the same time, transition metal ions only marginally reduced the amount of 2',3'-cGMP, blocking binase recognition sites of guanine at N7 of nucleophilic purine bases.
肠道微生物群的细胞外酶是影响身体功能活动的关键因素，因为它们直接与上皮细胞和免疫细胞相互作用。该属的几个物种，如细胞外核糖核酸酶binase的常见生产者，可作为定殖生物在肠道微生物群中繁殖。在不涉及金属离子作为辅助因子的情况下，binase通过切割3',5'-磷酸二酯键使RNA解聚，并在催化反应的第一阶段生成2',3'-环鸟苷磷酸。在反应混合物中保持一个多小时后，这些信使分子会影响人类肠道微生物群和人体。在本研究中，我们发现，在稳定RNA结构的过渡金属存在下，2',3'-cGMP的生成速率在增加。同时，过渡金属离子仅略微减少了2',3'-cGMP的量，阻断了亲核嘌呤碱基N7处鸟嘌呤的binase识别位点。
