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利用SSR标记对高羊茅进行遗传变异分析和品种鉴定的研究

Insight into the genetic variability analysis and cultivar identification of tall fescue by using SSR markers.

作者信息

Fu Kaixin, Guo Zhihui, Zhang Xinquan, Fan Yan, Wu Wendan, Li Daxu, Peng Yan, Huang Linkai, Sun Ming, Bai Shiqie, Ma Xiao

机构信息

Animal Science and Technology College, Sichuan Agricultural University, Chengdu, 611130 China.

Chongqing Municipal Institute of Animal Husbandry, Chongqing, 400039 China.

出版信息

Hereditas. 2016 Aug 11;153:9. doi: 10.1186/s41065-016-0013-1. eCollection 2016.

DOI:10.1186/s41065-016-0013-1
PMID:28096771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5226091/
Abstract

BACKGROUND

Genetic diversity of 19 forage-type and 2 turf-type cultivars of tall fescue ( Schreb.) was revealed using SSR markers in an attempt to explore the genetic relationships among them, and examine potential use of SSR markers to identify cultivars by bulked samples.

RESULTS

A total of 227 clear band was scored with 14 SSR primers and out of which 201 (88.6 %) were found polymorphic. The percentage of polymorphic bands (PPB) per primer pair varied from 62.5 to 100 % with an average of 86.9 %. The polymorphism information content (PIC) value ranged from 0.116 to 0.347 with an average of 0.257 and the highest PIC value (0.347) was noticed for primer NFA040 followed by NFA113 (0.346) whereas the highest discriminating power (D) of 1 was shown in NFA037 and LMgSSR02-01C. A Neighbor-joining dendrogram and the principal component analysis identified six major clusters and grouped the cultivars in agreement with their breeding histories. STRUCTURE analysis divided these cultivars into 3 sub-clades which correspond to distance based groupings.

CONCLUSION

These findings indicates that SSR markers by bulking strategy are a useful tool to measure genetic diversity among tall fescue cultivars and could be used to supplement morphological data for plant variety protection.

摘要

背景

利用SSR标记揭示了19个饲用型和2个草坪型高羊茅（Festuca arundinacea Schreb.）品种的遗传多样性，旨在探索它们之间的遗传关系，并检验SSR标记通过混合样本鉴定品种的潜在用途。

结果

14对SSR引物共检测到227条清晰条带，其中201条（88.6%）具有多态性。每对引物的多态性条带百分比（PPB）在62.5%至100%之间，平均为86.9%。多态性信息含量（PIC）值在0.116至0.347之间，平均为0.257，引物NFA040的PIC值最高（0.347），其次是NFA113（0.346），而NFA037和LMgSSR02 - 01C的判别能力（D）最高，为1。邻接法聚类图和主成分分析确定了六个主要聚类，并根据品种的育种历史对其进行了分组。STRUCTURE分析将这些品种分为3个亚分支，与基于距离的分组相对应。

结论

这些结果表明，通过混合策略使用SSR标记是衡量高羊茅品种间遗传多样性的有用工具，可用于补充植物品种保护的形态学数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee48/5226091/889d5431fda9/41065_2016_13_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee48/5226091/de470e2dac3e/41065_2016_13_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee48/5226091/889d5431fda9/41065_2016_13_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee48/5226091/de470e2dac3e/41065_2016_13_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee48/5226091/889d5431fda9/41065_2016_13_Fig2_HTML.jpg

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