Molecular Detection of spp. and spp. in Ruminants from Twelve Provinces of China.

spp. and spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China for spp. and spp. Positive samples were further analyzed with a standard PCR for the . spp. DNA was detected in the sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), and water buffaloes (6.9%; 2/29). spp. DNA was detected in sheep (1.8%; 2/111), goats (1.1%; 3/270), and cattle (3.6%; 65/1830) but not in water buffaloes (0/29). Sequencing of PCR products showed that , , , and sp. (JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also be , , sp. BL126-13 (KJ410243), and sp. JC3-6 (KM227012). Our study shows that domestic ruminants from China are not uncommonly infected with a variety of spp. and spp.