Alizadeh Rafieh, Navid Shadan, Abbasi Niloofar, Yari Abazar, Mazaheri Zohreh, Daneshi Erfan, Agarwal Ashok, Abbasi Mehdi
ENT and Head & Neck Research Center and Department, Hazrat Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2016 Dec;19(12):1279-1284. doi: 10.22038/ijbms.2016.7908.
Increased levels of nitric oxide (NO) in the testicular veins of people suffering from varicocele have already been reported. However, the role of NO-synthase (NOS) isozymes and their inhibitors have not been extensively studied. We aimed to evaluate the inhibitory effects of aminoguanidine (AG), on sperm motility, vitality, and mitochondrial membrane potential (MMP) in varicocelized rats.
Twenty fore male Wister rats were divided into control, sham, varicocele, and treatment groups. Varicocele and treatment groups underwent partial ligation of left renal vein. Rats in the sham group underwent the same procedures as the varicocele group with the exception of vein ligation. 10 weeks after varicocele induction, sperm parameters were evaluated in all groups. The treatment group received 50 mg/kg AG injection daily for 10 weeks after which they were sacrificed prior to assessment of the parameters. Sperm viability and MMP were assessed by flow cytometry using propidium iodide (PI) and rhodamine 123 (Rh123), respectively.
The results of this study show a decrease in sperm viability, motility and MMP in the varicocele group compared with the other groups. After AG injection, we observed that all the parameters were significantly enhanced in the treatment group compared with the other groups. Rh123 staining revealed a positive relation between MMP and sperm motility, whereas PI staining showed a positive relation between sperm motility and viability.
The findings of our study show that AG improves sperm motility and MMP, and thus, might be useful in the management of varicocele-related infertility.
已有报道称精索静脉曲张患者睾丸静脉中一氧化氮（NO）水平升高。然而，一氧化氮合酶（NOS）同工酶及其抑制剂的作用尚未得到广泛研究。我们旨在评估氨基胍（AG）对精索静脉曲张大鼠精子活力、存活率和线粒体膜电位（MMP）的抑制作用。
20只成年雄性Wistar大鼠分为对照组、假手术组、精索静脉曲张组和治疗组。精索静脉曲张组和治疗组行左肾静脉部分结扎术。假手术组除不进行静脉结扎外，其余操作与精索静脉曲张组相同。精索静脉曲张诱导10周后，评估所有组的精子参数。治疗组每天注射50mg/kg AG，持续10周，之后在评估参数前处死大鼠。分别使用碘化丙啶（PI）和罗丹明123（Rh123）通过流式细胞术评估精子活力和MMP。
本研究结果显示，与其他组相比，精索静脉曲张组的精子活力、运动能力和MMP均降低。注射AG后，我们观察到与其他组相比，治疗组的所有参数均显著提高。Rh123染色显示MMP与精子活力之间呈正相关，而PI染色显示精子活力与存活率之间呈正相关。
我们的研究结果表明，AG可提高精子活力和MMP，因此可能对精索静脉曲张相关不孕症的治疗有用。
