儿茶素、绿茶提取物和甲基黄嘌呤与庆大霉素联合对金黄色葡萄球菌和铜绿假单胞菌的作用：- 针对耐药菌的联合治疗。
Effect of Catechins, Green tea Extract and Methylxanthines in Combination with Gentamicin Against Staphylococcus aureus and Pseudomonas aeruginosa: - Combination therapy against resistant bacteria.
作者信息
Fazly Bazzaz Bibi Sedigheh, Sarabandi Sahar, Khameneh Bahman, Hosseinzadeh Hossein
机构信息
Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Students' Research Committee, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
出版信息
J Pharmacopuncture. 2016 Dec;19(4):312-318. doi: 10.3831/KPI.2016.19.032.
OBJECTIVES
Bacterial resistant infections have become a global health challenge and threaten the society's health. Thus, an urgent need exists to find ways to combat resistant pathogens. One promising approach to overcoming bacterial resistance is the use of herbal products. Green tea catechins, the major green tea polyphenols, show antimicrobial activity against resistant pathogens. The present study aimed to investigate the effect of catechins, green tea extract, and methylxanthines in combination with gentamicin against standard and clinical isolates of () and the standard strain of ().
METHODS
The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) values of different agents against bacterial strains were determined. The interactions of green tea extract, epigallate catechin, epigallocatechin gallate, two types of methylxanthine, caffeine, and theophylline with gentamicin were studied by using a checkerboard method and calculating the fraction inhibitory concentration index (FICI).
RESULTS
The MICs of gentamicin against bacterial strains were in the range of 0.312 - 320 μg/mL. The MIC values of both types of catechins were 62.5 - 250 μg/ mL. Green tea extract showed insufficient antibacterial activity when used alone. Methylxanthines had no intrinsic inhibitory activity against any of the bacterial strains tested. When green tea extract and catechins were combined with gentamicin, the MIC values of gentamicin against the standard strains and a clinical isolate were reduced, and synergistic activities were observed (FICI < 1). A combination of caffeine with gentamicin did not alter the MIC values of gentamicin.
CONCLUSION
The results of the present study revealed that green tea extract and catechins potentiated the antimicrobial action of gentamicin against some clinical isolates of and standard strains. Therefore, combinations of gentamicin with these natural compounds might be a promising approach to combat microbial resistance.
目的
细菌耐药性感染已成为一项全球性的健康挑战，威胁着社会健康。因此，迫切需要找到对抗耐药病原体的方法。一种有前景的克服细菌耐药性的方法是使用草药产品。绿茶儿茶素是绿茶中的主要多酚类物质，对耐药病原体具有抗菌活性。本研究旨在调查儿茶素、绿茶提取物和甲基黄嘌呤与庆大霉素联合使用对标准菌株和临床分离株（）以及标准菌株（）的影响。
方法
测定不同药物对细菌菌株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值。采用棋盘法并计算部分抑菌浓度指数（FICI），研究绿茶提取物、表没食子儿茶素、表没食子儿茶素没食子酸酯、两种甲基黄嘌呤、咖啡因和茶碱与庆大霉素的相互作用。
结果
庆大霉素对细菌菌株的MIC范围为0.312 - 320μg/mL。两种儿茶素的MIC值均为62.5 - 250μg/mL。单独使用时，绿茶提取物的抗菌活性不足。甲基黄嘌呤对所测试的任何细菌菌株均无内在抑制活性。当绿茶提取物和儿茶素与庆大霉素联合使用时，庆大霉素对标准菌株和一株临床分离株的MIC值降低，并观察到协同活性（FICI < 1）。咖啡因与庆大霉素联合使用未改变庆大霉素的MIC值。
结论
本研究结果表明，绿茶提取物和儿茶素可增强庆大霉素对某些临床分离株和标准菌株的抗菌作用。因此，庆大霉素与这些天然化合物联合使用可能是对抗微生物耐药性的一种有前景的方法。
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