Li Lu-Sheng, Yu Hao, Raynald Raynald, Wang Xiao-Dong, Dai Guang-Hui, Cheng Hong-Bin, Liu Xue-Bin, An Yi-Hua
Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University, Beijing, China; Department of Neurosurgery, Beijing Chao-yang Hospital Affiliated with Capital Medical University, China.
Department of Neurosurgery, Beijing Sanbo Brain Hospital, Capital Medical University , Beijing , China.
PeerJ. 2017 Jan 10;5:e2865. doi: 10.7717/peerj.2865. eCollection 2017.
The nerve fibre circuits around a lesion play a major role in the spontaneous recovery process after spinal cord hemisection in rats. The aim of the present study was to answer the following question: in the re-control process, do all spinal cord nerves below the lesion site participate, or do the spinal cord nerves of only one vertebral segment have a role in repair?
First we made a T7 spinal cord hemisection in 50 rats. Eight weeks later, they were divided into three groups based on distinct second operations at T7: ipsilateral hemisection operation, contralateral hemisection, or transection. We then tested recovery of hindlimbs for another eight weeks. The first step was to confirm the lesion had role or not in the spontaneous recovery process. Secondly, we performed T7 spinal cord hemisections in 125 rats. Eight weeks later, we performed a second single hemisection on the ipsilateral side at T8-T12 and then tested hindlimb recovery for another six weeks.
In the first part, the Basso, Beattie, Bresnahan (BBB) scores and the electrophysiology tests of both hindlimbs weren't significantly different after the second hemisection of the ipsilateral side. In the second part, the closer the second hemisection was to T12, the more substantial the resulting impairment in BBB score tests and prolonged latency periods.
The nerve regeneration from the lesion area after hemisection has no effect on spontaneous recovery of the spinal cord. Repair is carried out by all vertebrae caudal and ipsilateral to the lesion, with T12 being most important.
损伤周围的神经纤维回路在大鼠脊髓半切术后的自发恢复过程中起主要作用。本研究的目的是回答以下问题：在重新控制过程中，损伤部位以下的所有脊髓神经都参与其中，还是只有一个椎体节段的脊髓神经在修复中起作用？
首先，我们对50只大鼠进行T7脊髓半切术。八周后，根据在T7进行的不同第二次手术将它们分为三组：同侧半切术、对侧半切术或横断术。然后我们再测试八周的后肢恢复情况。第一步是确认损伤在自发恢复过程中是否起作用。其次，我们对125只大鼠进行T7脊髓半切术。八周后，我们在T8 - T12的同侧进行第二次单次半切术，然后再测试六周的后肢恢复情况。
在第一部分中，同侧第二次半切术后，双后肢的巴索、贝蒂、布雷斯纳汉（BBB）评分和电生理测试没有显著差异。在第二部分中，第二次半切术越靠近T12，BBB评分测试中的损伤就越严重，潜伏期延长也越明显。
半切术后损伤区域的神经再生对脊髓的自发恢复没有影响。修复是由损伤尾侧和同侧的所有椎骨进行的，其中T12最为重要。
