Brandão Pedro Francisco, Ramos Rui Miguel, Almeida Paulo Joaquim, Rodrigues José António
REQUIMTE/LAQV - Departamento de Quı́mica e Bioquı́mica, Faculdade de Ciências, Universidade do Porto , Rua do Campo Alegre no. 687, 4169-007 Porto, Portugal.
J Agric Food Chem. 2017 Feb 8;65(5):1037-1042. doi: 10.1021/acs.jafc.6b05370. Epub 2017 Jan 30.
A new approach is proposed for the extraction and determination of carbonyl compounds in solid samples, such as wood or cork materials. Cork products are used as building materials due to their singular characteristics; however, little is known about its aldehyde emission potential and content. Sample preparation was done by using a gas-diffusion microextraction (GDME) device for the direct extraction of volatile aldehydes and derivatization with 2,4-dinitrophenylhydrazine. Analytical determination of the extracts was done by HPLC-UV, with detection at 360 nm. The developed methodology proved to be a reliable tool for aldehyde determination in cork agglomerate samples with suitable method features. Mass spectrometry studies were performed for each sample, which enabled the identification, in the extracts, of the derivatization products of a total of 13 aldehydes (formaldehyde, acetaldehyde, furfural, propanal, 5-methylfurfural, butanal, benzaldehyde, pentanal, hexanal, trans-2-heptenal, heptanal, octanal, and trans-2-nonenal) and 4 ketones (3-hydroxy-2-butanone, acetone, cyclohexanone, and acetophenone). This new analytical methodology simultaneously proved to be consistent for the identification and determination of aldehydes in cork agglomerates and a very simple and straightforward procedure.
本文提出了一种用于提取和测定固体样品（如木材或软木材料）中羰基化合物的新方法。软木制品因其独特的特性而被用作建筑材料；然而，关于其醛类排放潜力和含量却知之甚少。样品制备采用气体扩散微萃取（GDME）装置直接提取挥发性醛类，并与2,4-二硝基苯肼进行衍生化反应。提取物的分析测定采用高效液相色谱-紫外检测法，检测波长为360nm。所开发的方法被证明是一种可靠的工具，用于测定软木团聚体样品中的醛类，具有合适的方法特性。对每个样品进行了质谱研究，这使得能够在提取物中鉴定出总共13种醛（甲醛、乙醛、糠醛、丙醛、5-甲基糠醛、丁醛、苯甲醛、戊醛、己醛、反式-2-庚烯醛、庚醛、辛醛和反式-2-壬烯醛）和4种酮（3-羟基-2-丁酮、丙酮、环己酮和苯乙酮）的衍生化产物。这种新的分析方法同时被证明对于软木团聚体中醛类的鉴定和测定是一致的，并且是一种非常简单直接的程序。
