An Young-Ji, Choi Seong-Jin, Kim Yong-Hyun, Lee Kyuhong
Department of Toxicology Evaluation, Konyang University, Daejeon, Republic of Korea.
Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup 56212, Republic of Korea.
J Anal Methods Chem. 2019 Apr 30;2019:3201370. doi: 10.1155/2019/3201370. eCollection 2019.
The quantitative analysis of target substances is an important part of assessing the toxicity of diverse materials. Usually, the quantitation of target compounds is conducted by instrumental analysis such as chromatography and capillary electrophoresis. If solvents are used in the pretreatment step of the target analyte quantification, it would be crucial to examine the solvent effect on the quantitative analysis. Therefore, in this study, we assessed the solvent effects using four different solvents (methanol, hexane, phosphate buffered saline (PBS), and dimethyl sulfoxide (DMSO)) and three toxic compounds (benzene, toluene, and methylisothiazolinone (MIT)). Liquid working standards containing the toxic compounds were prepared by dilution with each solvent and analyzed by gas chromatography-mass spectrometry (GC-MS). As a result, we found that the response factor (RF) values of the target analytes were different, depending on the solvent types. In particular, benzene and toluene exhibited their highest RF values (33,674 ng and 78,604 ng, respectively) in hexane, while the RF value of MIT was the highest (9,067 ng) in PBS. Considering the correlation ( ) and relative standard deviation (RSD) values, all target analytes showed fairly good values ( > 0.99 and RSD < 10%) in methanol and DMSO. In contrast, low (0.0562) and high RSD (10.6%) values of MIT were detected in hexane, while benzene and toluene exhibited relatively low and high RSD values in PBS (mean = 0.9892 ± 0.0146 and mean RSD = 13.3 ± 4.1%). Based on these findings, we concluded that the results and reliability of the quantitative analysis change depending on the analyte and solvent types. Therefore, in order to accurately assess the toxicity of target compounds, reliable analytical data should be obtained, preferentially by considering the solvent types.
目标物质的定量分析是评估多种材料毒性的重要组成部分。通常，目标化合物的定量分析通过色谱法和毛细管电泳等仪器分析方法进行。如果在目标分析物定量的预处理步骤中使用了溶剂，那么研究溶剂对定量分析的影响就至关重要。因此，在本研究中，我们使用四种不同的溶剂（甲醇、己烷、磷酸盐缓冲盐水（PBS）和二甲基亚砜（DMSO））以及三种有毒化合物（苯、甲苯和甲基异噻唑啉酮（MIT））来评估溶剂效应。通过用每种溶剂稀释制备含有毒化合物的液体工作标准品，并采用气相色谱 - 质谱联用（GC - MS）进行分析。结果发现，目标分析物的响应因子（RF）值因溶剂类型而异。特别是，苯和甲苯在己烷中表现出最高的RF值（分别为33,674 ng和78,604 ng），而MIT在PBS中的RF值最高（9,067 ng）。考虑到相关性（ ）和相对标准偏差（RSD）值，所有目标分析物在甲醇和DMSO中均显示出相当好的值（ ＞0.99且RSD＜10%）。相比之下，在己烷中检测到MIT的低 （0.0562）和高RSD（10.6%）值，而苯和甲苯在PBS中表现出相对较低的 和较高的RSD值（平均 ＝0.9892±0.0146且平均RSD＝13.3±4.1%）。基于这些发现，我们得出结论，定量分析的结果和可靠性会因分析物和溶剂类型的不同而变化。因此，为了准确评估目标化合物的毒性，应优先考虑溶剂类型以获得可靠的分析数据。
