Çil N, Oğuz E O, Mete E, Çetinkaya A, Mete G A
a Departments of Histology and Embryology.
b Departments of Microbiology, Faculty of Medicine , Pamukkale University , Denizli , Turkey.
Biotech Histochem. 2017;92(1):15-28. doi: 10.1080/10520295.2016.1243728. Epub 2017 Jan 18.
The use of stem or progenitor cells from bone marrow, or peripheral or umbilical cord blood is becoming more common for treatment of diabetic foot problems. These cells promote neovascularization by angiogenic factors and they promote epithelium formation by stimulating cell replication and migration under certain pathological conditions. We investigated the role of CD34 + stem cells from human umbilical cord blood in wound healing using a rat model. Rats were randomly divided into a control group and two groups with diabetes induced by a single dose of 55 mg/kg intraperitoneal streptozocin. Scarred areas 5 mm in diameter were created on the feet of all rats. The diabetic rats constituted the diabetes control group and a diabetes + stem cell group with local injection into the wound site of 0.5 × 106 CD34 + stem cells from human umbilical cord blood. The newly formed skin in the foot wounds following CD34 + stem cell treatment showed significantly improvement by immunohistochemistry and TUNEL staining, and were closer to the wound healing of the control group than the untreated diabetic animals. The increase in FGF expression that accompanied the local injection of CD34 + stem cells indicates that FGF stimulation helped prevent apoptosis. Our findings suggest a promising new treatment approach to diabetic wound healing.
使用来自骨髓、外周血或脐带血的干细胞或祖细胞来治疗糖尿病足问题正变得越来越普遍。这些细胞通过血管生成因子促进新血管形成，并且在某些病理条件下通过刺激细胞复制和迁移来促进上皮形成。我们使用大鼠模型研究了人脐带血中CD34 +干细胞在伤口愈合中的作用。大鼠被随机分为对照组和两组，通过腹腔注射单剂量55 mg/kg链脲佐菌素诱导糖尿病。在所有大鼠的足部制造直径5 mm的瘢痕区域。糖尿病大鼠构成糖尿病对照组和糖尿病+干细胞组，将0.5×106个人脐带血CD34 +干细胞局部注射到伤口部位。通过免疫组织化学和TUNEL染色，CD34 +干细胞治疗后足部伤口新形成的皮肤显示出明显改善，并且与未治疗的糖尿病动物相比，更接近对照组的伤口愈合。局部注射CD34 +干细胞伴随的FGF表达增加表明FGF刺激有助于防止细胞凋亡。我们的研究结果提示了一种有前景的糖尿病伤口愈合新治疗方法。
