Department of Integrative Physiology and Pathobiology, Tufts University School of Medicine , Boston, Massachusetts 02111, United States.
The Valspar Corporation , Packaging Division, Sewickley, Pennsylvania 15143, United States.
Environ Sci Technol. 2017 Feb 7;51(3):1718-1726. doi: 10.1021/acs.est.6b04704. Epub 2017 Jan 18.
Consumer concerns about exposure to substances found in food contact materials with estrogenic activity (EA) have created substantial demand for alternatives. We assessed the potential EA of both a new bisphenol monomer used to synthesize polymeric coatings for metal food-contact applications and the nonintentionally added substances (NIAS) that may migrate into food. We evaluated tetramethyl bisphenol F (TMBPF) using in vitro and in vivo assays. We extracted the polymeric coating using food simulants ethanol (50% v/v) and acetic acid (3% w/v) and measured migration using tandem liquid chromatography (LC)/mass spectrometry (MS) and LC time-of-flight MS for TMBPF and NIAS, respectively. We also tested migrants for EA using the E-SCREEN assay. TMBPF did not show estrogenic activity in the uterotrophic assay and did not alter puberty in male and female rats or mammary gland development in female rats. Neither TMBPF nor the migrants from the final polymeric coating increased proliferation of estrogen-sensitive MCF7 cells. TMBPF did not show estrogen-agonist or antagonist activity in the estrogen receptor-transactivation assay. TMBPF migration was below the 0.2 parts per billion detection limit. Our findings provide compelling evidence for the absence of EA by TMBPF and the polymeric coating derived from it and that human exposure to TMBPF would be negligible.
消费者对接触具有雌激素活性（EA）的食品接触材料的物质的担忧，导致对替代品的大量需求。我们评估了一种新的双酚单体的潜在 EA，该单体用于合成用于金属食品接触应用的聚合涂层，以及可能迁移到食品中的非故意添加物质（NIAS）。我们使用体外和体内测定法评估了四甲基双酚 F（TMBPF）。我们使用食品模拟物乙醇（50% v/v）和乙酸（3% w/v）提取聚合涂层，并使用串联液相色谱（LC）/质谱（MS）和 LC 飞行时间 MS 分别测量 TMBPF 和 NIAS 的迁移量。我们还使用 E-SCREEN 测定法测试了 EA 迁移物。TMBPF 在子宫增重测定中没有表现出雌激素活性，也没有改变雄性和雌性大鼠的青春期或雌性大鼠的乳腺发育。TMBPF 或最终聚合涂层中的迁移物都没有增加雌激素敏感的 MCF7 细胞的增殖。TMBPF 在雌激素受体转激活测定中没有表现出雌激素激动剂或拮抗剂活性。TMBPF 的迁移量低于 0.2 十亿分之的检测限。我们的研究结果提供了令人信服的证据，证明 TMBPF 和源自它的聚合涂层没有 EA，并且人类接触 TMBPF 将是微不足道的。
