Lee Heung-Shick, Kim Younhee
Department of Biotechnology and Bioinformatics, Korea University, Sejongsi 30019, Republic of Korea.
Department of Korean Medicine, Semyung University, Jecheon 27136, Republic of Korea.
J Microbiol Biotechnol. 2017 Feb 28;27(2):395-404. doi: 10.4014/jmb.1611.11064.
Fungal cell walls and cell membranes are the main targets of antifungals. In this study, we report on the antifungal activity of an ethanol extract from against , showing that the antifungal activity is associated with the synergistic actions of preventing cell wall synthesis, enabling membrane depolarization, and compromising permeability. First, it was shown that the ethanol extract from was involved in damaging the integrity of cell walls in . In isotonic media, cell bursts of by the ethanol extract could be restored, and the minimum inhibitory concentration (MIC) of the ethanol extract against cells increased 4-fold. In addition, synthesis of (1,3)-β--glucan polymer was inhibited by 87% and 83% following treatment of microsomes with the ethanol extract at their 1× MIC and 2× MIC, respectively. Second, the ethanol extract from influenced the function of cell membranes. cells treated with the ethanol extract formed red aggregates by staining with a membrane-impermeable dye, propidium iodide. Membrane depolarization manifested as increased fluorescence intensity by staining -treated cells with a membrane-potential marker, DiBAC(3) ((-1,3-dibutylbarbituric acid) trimethine oxonol). Membrane permeability was assessed by crystal violet assay, and cells treated with the ethanol extract exhibited significant uptake of crystal violet in a concentration-dependent manner. The findings suggest that ethanol extract is a viable and effective candidate for the development of new antifungal agents to treat -associated diseases.
真菌细胞壁和细胞膜是抗真菌药物的主要作用靶点。在本研究中，我们报告了[具体提取物]乙醇提取物对[具体真菌]的抗真菌活性，表明其抗真菌活性与阻止细胞壁合成、使细胞膜去极化以及损害通透性的协同作用相关。首先，研究表明[具体提取物]的乙醇提取物会破坏[具体真菌]细胞壁的完整性。在等渗培养基中，[具体真菌]被[具体提取物]乙醇提取物导致的细胞破裂可以恢复，并且该乙醇提取物对[具体真菌]细胞的最低抑菌浓度（MIC）增加了4倍。此外，用其1×MIC和2×MIC的[具体提取物]乙醇提取物处理[具体真菌]微粒体后，（1,3）-β-D-葡聚糖聚合物的合成分别被抑制了87%和83%。其次，[具体提取物]的乙醇提取物影响了[具体真菌]细胞膜的功能。用[具体提取物]乙醇提取物处理的[具体真菌]细胞经膜不可渗透染料碘化丙啶染色后形成红色聚集体。用膜电位标记物DiBAC(3)（（-1,3-二丁基巴比妥酸）三甲川草酚菁）对[具体提取物]处理的[具体真菌]细胞染色，膜去极化表现为荧光强度增加。通过结晶紫测定法评估膜通透性，用[具体提取物]乙醇提取物处理的[具体真菌]细胞呈现出浓度依赖性的显著结晶紫摄取。这些发现表明，[具体提取物]乙醇提取物是开发用于治疗与[具体真菌]相关疾病的新型抗真菌药物的可行且有效的候选物。
