Medical Mycology Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringá, Colombo Avenue, 5790, Maringá, PR, Brazil; Graduate Program in Health Sciences, State University of Maringá, Colombo Avenue, 5790, Maringá, PR, Brazil.
Medical Mycology Laboratory, Department of Clinical Analysis and Biomedicine, State University of Maringá, Colombo Avenue, 5790, Maringá, PR, Brazil.
J Ethnopharmacol. 2020 Jun 28;256:112791. doi: 10.1016/j.jep.2020.112791. Epub 2020 Mar 28.
The use of natural products such as propolis extract (PE) is a promising alternative when topically administered to replace conventional antifungals, mostly due to its therapeutic applications, ease of access and low toxicity. However, despite being the subject of several mycology studies, they focus primarily on exploiting their antimicrobial activity, lacking information on the mechanisms of action of PE on Candida spp., characterizing its antifungal potential.
To elucidate the bioactivity of PE on the cellular structure of Candida albicans.
A total of seven C. albicans clinical isolates plus a reference strain of C. albicans ATCC 90028 were used in this study. The PE was characterized and its effect on C. albicans was determined by susceptibility and growth kinetics assays; interference on C. albicans germination and filamentation; evaluation of the integrity of the C. albicans cell wall and membrane, as well as its mutagenic potential.
The PE presented strong inhibitory activity, which showed its greatest antifungal activity at 12 h with dose and time dependent fungistatic characteristics, effectively inhibiting and interfering on C. albicans filamentation. In addition, PE caused membrane and cell wall damage with intracellular content extravasation. Moreover, PE was not mutagenic.
The bioactivity of PE is mainly related to the loss of integrity membrane as well as the integrity of the cell wall and consequent increase in permeability, without mutagenic effects.
当局部应用以替代常规抗真菌药物时，使用天然产物如蜂胶提取物（PE）是一种很有前途的替代方法，这主要是由于其治疗应用、易于获得和低毒性。然而，尽管它是几项真菌学研究的主题，但这些研究主要集中在利用其抗菌活性上，缺乏关于 PE 对念珠菌属作用机制的信息，因此无法确定其抗真菌潜力。
阐明 PE 对白色念珠菌细胞结构的生物活性。
本研究共使用了 7 株白色念珠菌临床分离株和一株白色念珠菌 ATCC 90028 参考菌株。对 PE 进行了表征，并通过药敏和生长动力学测定、对白色念珠菌发芽和菌丝形成的干扰、评估白色念珠菌细胞壁和膜的完整性以及其致突变潜力来确定其对白色念珠菌的影响。
PE 表现出很强的抑制活性，在 12 小时内显示出最大的抗真菌活性，具有剂量和时间依赖性的抑菌特性，能有效抑制和干扰白色念珠菌的菌丝形成。此外，PE 会导致细胞膜和细胞壁损伤，使细胞内容物外渗。此外，PE 没有致突变作用。
PE 的生物活性主要与膜的完整性丧失以及细胞壁的完整性和随之而来的通透性增加有关，没有致突变作用。
