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等离子体活化的“樟蜂醋剂”作为创新型抗真菌剂及其灭活机制

Plasma activated Ezhangfeng Cuji as innovative antifungal agent and its inactivation mechanism.

作者信息

Lin Lin, Zhuo Yue, Dong Qiran, Yang Chunjun, Cheng Cheng, Liu Taofeng

机构信息

The Postgraduate School of Anhui, University of Chinese Medicine, Hefei, 230012, People's Republic of China.

Department of Dermatology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, 230012, People's Republic of China.

出版信息

AMB Express. 2023 Jun 27;13(1):65. doi: 10.1186/s13568-023-01571-6.

DOI:10.1186/s13568-023-01571-6
PMID:37368076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10299983/
Abstract

Candida albicans is a highly drug-resistant fungus for which new treatments are urgently needed due to the lack of clinically effective options. In this study, we evaluated the antifungal activity and mechanism of plasma-activated Ezhangfeng Cuji (PAEC) against Candida albicans and compared it with physiological saline (PS), plasma-activated physiological saline (PAPS) and Ezhangfeng Cuji (EC). After dielectric barrier discharge (DBD) plasma treatment with EC for 20 min followed by a 10 min immersion of Candida albicans, the fungus was reduced by approximately 3 orders of magnitude. High performance liquid chromatography (HPLC) results showed an increase of 41.18% and 129.88% in the concentration of oxymatrine and rhein, respectively, after plasma-treated EC. The concentrations of reactive species (RS), such as HO, [Formula: see text], and O, were found to be higher and the pH value was getting lower in PS after plasma treatment. Detailed analysis of intracellular material leakage, reactive oxygen species (ROS), apoptosis for Candida albicans and observation by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) demonstrated that PAPS, EC and PAEC disrupt the morphological structure of Candida albicans to varying degrees.Additionally, specific analyses on Candida albicans virulence factors, such as adhesion to tissue surfaces, cell surface hydrophobicity (CSH), the transition of yeast-phase cells to mycelium-phase cells, and the secretion of hydrolytic enzymes for Candida albicans were conducted and found to be inhibited after PAPS/EC/PAEC treatment. In our investigation, the inhibitory effects on Candida albicans were ranked from strong to weak as follows: PAEC, EC, PAPS, and PS.

摘要

白色念珠菌是一种高度耐药的真菌，由于缺乏临床有效的治疗选择，迫切需要新的治疗方法。在本研究中，我们评估了等离子体活化的莪冰草本抑菌剂（PAEC）对白色念珠菌的抗真菌活性和作用机制，并将其与生理盐水（PS）、等离子体活化生理盐水（PAPS）和莪冰草本抑菌剂（EC）进行了比较。在用EC进行介质阻挡放电（DBD）等离子体处理20分钟后，将白色念珠菌浸泡10分钟，真菌数量减少了约3个数量级。高效液相色谱（HPLC）结果显示，等离子体处理后的EC中氧化苦参碱和大黄酸的浓度分别增加了41.18%和129.88%。等离子体处理后，PS中活性物种（RS）如HO、[公式：见原文]和O的浓度较高，pH值降低。对白色念珠菌的细胞内物质泄漏、活性氧（ROS）、凋亡进行详细分析，并通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）观察，结果表明PAPS、EC和PAEC对白色念珠菌的形态结构有不同程度的破坏。此外，还对白色念珠菌的毒力因子进行了特异性分析，如对组织表面的粘附、细胞表面疏水性（CSH）、酵母相细胞向菌丝相细胞的转变以及白色念珠菌水解酶的分泌，发现PAPS/EC/PAEC处理后这些毒力因子受到抑制。在我们的研究中，对白色念珠菌的抑制作用从强到弱依次为：PAEC、EC、PAPS和PS。

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