Deng Chuncao, Song Feng, Li Jienan, Ye Yi, Zhang Lushun, Liang Weibo, Luo Haibo, Li Yingbi
Department of Forensic Genetics, West China School of Preclinical and Forensic Medicine, Sichuan University, 3-17 Renmin South Road, Chengdu, Sichuan, 610041, People's Republic of China.
Department of Forensic Analytical Toxicology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041, China.
Int J Legal Med. 2017 Jul;131(4):975-977. doi: 10.1007/s00414-017-1538-1. Epub 2017 Jan 18.
Application of X-STRs as complements of autosomal STR application in the forensic genetics has become a tendency for kinship testing, especially in deficiency paternity cases. Recently, a novel kit of 19 X-STR loci was developed, which permitted the analysis of 19 STR in the same PCR reaction, and these markers can be clustered into seven groups for the physical linkage. The objective of this study was to evaluate the allele and haplotype diversity of 19 X-STR loci in the Uygur (n = 220) and Tibetan nationality (n = 270) and to estimate the usefulness for complex kinship analysis. In the Tibetan and Uygur populations, a total of alleles of all loci were 188 and 212, with the allele frequencies ranged from 0.0037 to 0.5593 and from 0.0045 to 0.5409, respectively. Compared with previous studies, DXS10135 was the most polymorphic locus in the two population groups, whereas the least variant locus was DXS10164 in the Uygur population and DXS7423 in the Tibetan nationality. Haplotype diversity obtained in this investigation was greater than 0.9 across all LGs. This study indicated the new kit could be used as a supplementary tool in kinship testing in China. In addition, the data sets can be used as supplementary national X-STR references to enlarge the database.
在法医遗传学中，应用X染色体短串联重复序列（X-STRs）作为常染色体STR应用的补充已成为亲缘关系检测的一种趋势，尤其是在父子关系缺失的案件中。最近，开发了一种包含19个X-STR位点的新型试剂盒，它允许在同一聚合酶链反应（PCR）中分析19个STR，并且这些标记可聚类为7组用于物理连锁分析。本研究的目的是评估19个X-STR位点在维吾尔族（n = 220）和藏族（n = 270）中的等位基因和单倍型多样性，并估计其在复杂亲缘关系分析中的实用性。在藏族和维吾尔族人群中，所有位点的等位基因总数分别为188个和212个，等位基因频率分别为0.0037至0.5593和0.0045至0.5409。与先前的研究相比，DXS10135是两个人群组中多态性最高的位点，而维吾尔族人群中变异最少的位点是DXS10164，藏族人群中是DXS7423。本研究中获得的所有连锁群（LG）的单倍型多样性均大于0.9。本研究表明，该新型试剂盒可作为中国亲缘关系检测的补充工具。此外，这些数据集可作为国家X-STR补充参考数据以扩大数据库。
