He Guanglin, Li Ye, Zou Xing, Zhang Ying, Li Hepei, Wang Mengge, Wu Jin
Department of Forensic Genetics, West China School of Basic Sciences and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu, 610041, Sichuan, People's Republic of China.
Department of Forensic Medicine, College of Basic Medicine, Chongqing Medical University, Chongqing, 400042, China.
Int J Legal Med. 2018 Mar;132(2):409-413. doi: 10.1007/s00414-017-1672-9. Epub 2017 Sep 3.
The X-chromosomal short tandem repeats (STRs) with more informative than autosomal STRs in some complicated biological relationships identification due to its specific mode of genetic transmission can be used as a complementary tool in forensic case practices. In this study, we presented the population genetic data of 19 X-STRs, consisting of DXS10174, DXS10075, DXS10079, DXS101, DXS10101, DXS10103, DXS10134, DXS10135, DXS10148, DXS10159, DXS10162, DXS10164, DXS6789, DXS6809, DXS7132, DXS7423, DXS7424, DXS8378, and HPRTB loci, in a sample of 235 individuals of Tibetan nationality from Sichuan province, Southwest China. All 19 X-STR loci were consistent with Hardy-Weinberg equilibrium. The results showed that the combined power of discrimination in females and males are 0.999999999999999999997 and 0.9999999999997, respectively. In addition, the mean paternity exclusion chances based on the formula of MEC, MEC, and MEC as well as MEC are 0.99999991, 0.9999999999924, 0.9999999999929, and 0.999999985, respectively. In summary, our findings suggested that the AGCU X19 kit can be considered to serve as a high polymorphic information tool for forensic identification and kinship testing in the Sichuan Tibetan population. Furthermore, population genetic structure investigation between Sichuan Tibetan population and other 19 populations using PCA, MDS, and phylogenetic tree illustrated that significant genetic difference was observed between the Sichuan Tibetan and Malay, as well as the Xinjiang Uyghur population.
X染色体短串联重复序列（STRs）因其特定的遗传传递模式，在一些复杂生物学关系鉴定中比常染色体STRs更具信息性，可作为法医案件实践中的一种补充工具。在本研究中，我们呈现了来自中国西南部四川省的235名藏族个体样本中19个X-STRs的群体遗传数据，这些X-STRs包括DXS10174、DXS10075、DXS10079、DXS101、DXS10101、DXS10103、DXS10134、DXS10135、DXS10148、DXS10159、DXS10162、DXS10164、DXS6789、DXS6809、DXS7132、DXS7423、DXS7424、DXS8378和HPRTB基因座。所有19个X-STR基因座均符合哈迪-温伯格平衡。结果显示，女性和男性的联合鉴别力分别为0.999999999999999999997和0.9999999999997。此外，基于MEC公式、MEC、MEC以及MEC的平均父权排除机会分别为0.99999991、0.9999999999924、0.9999999999929和0.999999985。总之，我们的研究结果表明，AGCU X19试剂盒可被视为四川藏族人群法医鉴定和亲属关系检测的高多态信息工具。此外，使用主成分分析（PCA）、多维尺度分析（MDS）和系统发育树对四川藏族人群与其他19个群体之间的群体遗传结构进行调查表明，四川藏族与马来人群以及新疆维吾尔族人群之间存在显著的遗传差异。
