Mu Chunlong, Yang Yuxiang, Yu Kaifan, Yu Miao, Zhang Chuanjian, Su Yong, Zhu Weiyun
Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Amino Acids. 2017 Apr;49(4):771-781. doi: 10.1007/s00726-017-2379-4. Epub 2017 Jan 18.
In-feed antibiotics have been used to promote growth in piglets, but its impact on metabolomics profiles associated with host metabolism is largely unknown. In this study, to test the hypothesis that antibiotic treatment may affect metabolite composition both in the gut and host biofluids, metabolomics profiles were analyzed in antibiotic-treated piglets. Piglets were fed a corn-soy basal diet with or without in-feed antibiotics from postnatal day 7 to day 42. The serum biochemical parameters, metabolomics profiles of the serum, urine, and jejunal digesta, and indicators of microbial metabolism (short-chain fatty acids and biogenic amines) were analyzed. Compared to the control group, antibiotics treatment did not have significant effects on serum biochemical parameters except that it increased (P < 0.05) the concentration of urea. Antibiotics treatment increased the relative concentrations of metabolites involved in amino-acid metabolism in the serum, while decreased the relative concentrations of most amino acids in the jejunal content. Antibiotics reduced urinary 2-ketoisocaproate and hippurate. Furthermore, antibiotics decreased (P < 0.05) the concentrations of propionate and butyrate in the feces. Antibiotics significantly affected the concentrations of biogenic amines, which are derived from microbial amino-acid metabolism. The three major amines, putrescine, cadaverine, and spermidine, were all increased (P < 0.05) in the large intestine of antibiotics-treated piglets. These results identified the phenomena that in-feed antibiotics may have significant impact on the metabolomic markers of amino-acid metabolism in piglets.
饲料中添加抗生素已被用于促进仔猪生长，但其对与宿主代谢相关的代谢组学特征的影响尚不清楚。在本研究中，为了验证抗生素治疗可能影响肠道和宿主生物体液中代谢物组成这一假设，对接受抗生素治疗的仔猪进行了代谢组学分析。从出生后第7天到第42天，给仔猪喂食含或不含饲料中添加抗生素的玉米-大豆基础日粮。分析了血清生化参数、血清、尿液和空肠消化物的代谢组学特征以及微生物代谢指标（短链脂肪酸和生物胺）。与对照组相比，抗生素治疗对血清生化参数没有显著影响，只是尿素浓度有所升高（P<0.05）。抗生素治疗增加了血清中参与氨基酸代谢的代谢物的相对浓度，而降低了空肠内容物中大多数氨基酸的相对浓度。抗生素降低了尿中2-酮异己酸和马尿酸盐的含量。此外，抗生素降低了（P<0.05）粪便中丙酸和丁酸的浓度。抗生素显著影响了源自微生物氨基酸代谢的生物胺的浓度。在接受抗生素治疗的仔猪的大肠中，三种主要胺类，腐胺、尸胺和亚精胺的含量均有所增加（P<0.05）。这些结果表明，饲料中添加抗生素可能对仔猪氨基酸代谢的代谢组学标志物有显著影响。
