Balestrini Raffaella, Salvioli Alessandra, Dal Molin Alessandra, Novero Mara, Gabelli Giovanni, Paparelli Eleonora, Marroni Fabio, Bonfante Paola
Istituto per la Protezione Sostenibile delle Piante del CNR, SS Torino, Viale P.A. Mattioli 25, 10125, Torino, Italy.
Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università degli Studi di Torino, Viale P.A. Mattioli 25, 10125, Torino, Italy.
Mycorrhiza. 2017 Jul;27(5):417-430. doi: 10.1007/s00572-016-0754-8. Epub 2016 Dec 27.
Grapevine, cultivated for both fruit and beverage production, represents one of the most economically important fruit crops worldwide. With the aim of better understanding how grape roots respond to beneficial microbes, a transcriptome sequencing experiment has been performed to evaluate the impact of a single arbuscular mycorrhizal (AM) fungal species (Funneliformis mosseae) versus a mixed inoculum containing a bacterial and fungal consortium, including different AM species, on Richter 110 rootstock. Results showed that the impact of a single AM fungus and of a complex microbial inoculum on the grapevine transcriptome differed. After 3 months, roots exclusively were colonized after the F. mosseae treatment and several AM marker genes were found to be upregulated. The mixed inoculum led only to traces of colonization by AM fungi, but elicited an important transcriptional regulation. Additionally, the expression of genes belonging to categories such as nutrient transport, transcription factors, and cell wall-related genes was significantly altered in both treatments, but the exact genes affected differed in the two conditions. These findings advance our understanding about the impact of soil beneficial microbes on the root system of a woody plant, also offering the basis for novel approaches in grapevine cultivation.
葡萄，因其果实和用于饮料生产而被种植，是全球经济上最重要的水果作物之一。为了更好地了解葡萄根系对有益微生物的反应，进行了一项转录组测序实验，以评估单一丛枝菌根（AM）真菌物种（摩西斗管囊霉）与包含细菌和真菌联合体（包括不同AM物种）的混合接种物对里扎马特110砧木的影响。结果表明，单一AM真菌和复杂微生物接种物对葡萄转录组的影响不同。3个月后，摩西斗管囊霉处理后仅根系被定殖，并且发现几个AM标记基因上调。混合接种物仅导致AM真菌定殖的痕迹，但引发了重要的转录调控。此外，在两种处理中，属于营养运输、转录因子和细胞壁相关基因等类别的基因表达均发生了显著变化，但在两种情况下受影响的具体基因不同。这些发现增进了我们对土壤有益微生物对木本植物根系影响的理解，也为葡萄栽培的新方法提供了基础。
