Adachi Shin-Ichi, Nihei Ken-Ichi, Ishihara Yoshiyuki, Yoshizawa Fumiaki, Yagasaki Kazumi
Center for Bioscience Research and Education, Utsunomiya University, Tochigi, 321-8505, Japan.
Faculty of Agriculture, Utsunomiya University, Tochigi, 321-8505, Japan.
Cytotechnology. 2017 Apr;69(2):329-336. doi: 10.1007/s10616-016-0061-4. Epub 2017 Jan 18.
Hyperuricemia is recognized as an important risk factor for gout. High dietary intake of purine-rich foods such as meats and sea foods increases uric acid (UA) levels in the blood. Taxifolin present in Siberian larch and strawberries has been reported to possess health promoting activities including anti-oxidant effect. In this study, we examined anti-hyperuricemic effect of taxifolin in both cultured hepatocytes and hyperuricemic model mice. In cultured AML12 hepatocytes, taxifolin significantly suppressed UA production dose- and time-dependently. In mice with hyperuricemia induced by concurrent administration of guanosine-5'-monophosphate and inosine-5'-monophosphate, oral administration of taxifolin suppressed the increases in plasma and liver UA levels. In addition, it also suppressed hepatic xanthine oxidase (XO) activity. Thus, anti-hyperuricemic effect of taxifolin could be explained, at least partly, by suppressing UA production via inhibition of XO activity in the liver. These results suggest that taxifolin possesses a potent hypouricemic effect and it could be a potential candidate for an anti-hyperuricemic phytochemical.
高尿酸血症被认为是痛风的一个重要危险因素。高膳食摄入富含嘌呤的食物，如肉类和海鲜，会增加血液中的尿酸（UA）水平。据报道，西伯利亚落叶松和草莓中含有的紫杉叶素具有促进健康的活性，包括抗氧化作用。在本研究中，我们研究了紫杉叶素在培养的肝细胞和高尿酸血症模型小鼠中的抗高尿酸血症作用。在培养的AML12肝细胞中，紫杉叶素剂量和时间依赖性地显著抑制尿酸生成。在同时给予5'-单磷酸鸟苷和5'-单磷酸肌苷诱导的高尿酸血症小鼠中，口服紫杉叶素可抑制血浆和肝脏尿酸水平的升高。此外，它还抑制肝脏黄嘌呤氧化酶（XO）活性。因此，紫杉叶素的抗高尿酸血症作用至少部分可以通过抑制肝脏中XO活性来抑制尿酸生成来解释。这些结果表明，紫杉叶素具有强大的降尿酸作用，它可能是一种潜在的抗高尿酸血症植物化学物质候选物。
