Mioduchowska Monika, Kaczmarczyk Agnieszka, Zając Katarzyna, Zając Tadeusz, Sell Jerzy
Department of Genetics, University of Gdansk, 80-308 Gdansk, Poland.
Institute of Nature Conservation, Polish Academy of Sciences, 31-120 Krakow, Poland.
J Exp Zool A Ecol Genet Physiol. 2016 Nov;325(9):610-625. doi: 10.1002/jez.2055.
Some bivalve species possess two independent mitochondrial DNA lineages: maternally (F-type) and paternally (M-type) inherited. This phenomenon is called doubly uniparental inheritance. It is generally agreed that F-type mtDNA is typically present in female somatic and gonadal tissues as well as in male somatic tissues, whereas the M-type mtDNA occurs only in male germ line and gonadal tissue. In the present study, the mtDNA heteroplasmy (for both F and M genomes) in male somatic tissues of Unio crassus (Philipsson, 1788), species threatened with extinction, has been confirmed. Taking advantage from the presence of Mcox1 marker only in male somatic tissues, we developed a new method of sex identification in this endangered species, using nondestructive tissue sampling. Probability of correct sex identification was estimated at 97.5%. The present study is the first report on gender-associated mitochondrial DNA heteroplasmy in male somatic tissues of thick-shelled river mussel and first approach to U. crassus sex identification at molecular level. Our study also confirmed the utility of paternally inherited Mcox1 gene fragment as a complementary molecular tool for resolving phylogeographical relationships among populations of thick-shelled river mussel.
一些双壳类物种拥有两个独立的线粒体DNA谱系：母系遗传（F型）和父系遗传（M型）。这种现象被称为双单亲遗传。普遍认为，F型线粒体DNA通常存在于雌性体细胞和性腺组织以及雄性体细胞组织中，而M型线粒体DNA仅存在于雄性生殖系和性腺组织中。在本研究中，已证实濒危物种厚壳河蚌（菲利普松，1788年）雄性体细胞组织中存在线粒体DNA异质性（F和M基因组均有）。利用仅在雄性体细胞组织中存在的Mcox1标记，我们开发了一种在这种濒危物种中进行性别鉴定的新方法，采用非破坏性组织取样。正确性别鉴定的概率估计为97.5%。本研究是关于厚壳河蚌雄性体细胞组织中与性别相关的线粒体DNA异质性的首次报告，也是在分子水平上对厚壳河蚌进行性别鉴定的首次尝试。我们的研究还证实了父系遗传的Mcox1基因片段作为解决厚壳河蚌种群间系统发育关系的补充分子工具的实用性。
